DNA repair after ultraviolet irradiation of ICR 2A frog cells. Pyrimidine dimers are long acting blocks to nascent DNA synthesis

Biophysical Journal
B S Rosenstein, R B Setlow

Abstract

The ability of ICR 2A frog cells to repair DNA damage induced by ultraviolet irradiation was examined. These cells are capable of photoreactivation but are nearly totally deficient in excision repair. They have the ability to convert the small molecule weight DNA made after irradiation into large molecules but do not show an enhancement in this process when the UV dose is delivered in two separate exposures separated by a 3- or 24-h incubation. Total DNA synthesis is depressed and low molecular weight DNA continues to be synthesized during pulse-labeling as long as 48 h after irradiation. The effects of pyrimidine dimer removal through exposure of UV irradiated cells to photoreactivating light indicate that dimers act as the critical lesions blocking DNA synthesis.

Citations

Jan 1, 1982·Molecular & General Genetics : MGG·R E Calza, A L Schroeder
Jan 1, 1982·Molecular & General Genetics : MGG·R E Calza, A L Schroeder
Jun 1, 1995·Journal of Photochemistry and Photobiology. B, Biology·B S Rosenstein, C A Vaslet
Mar 22, 2002·Mutation Research·Richard B Setlow
Aug 1, 1981·Quarterly Reviews of Biophysics·R MeneghiniR I Schumacher
Aug 29, 2002·The EMBO Journal·Wouter SchulGijsbertus T J van der Horst
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