PMID: 7335106Dec 1, 1981Paper

DNA repair in a UV-sensitive mutant of a mouse cell line

Mutation Research
K Sato, R B Setlow

Abstract

A UV-sensitive mutant, Q31, isolated from mouse-lymphoma L5178Y cells, was studied for excision and post-replication repairs. A nearly equal number of UV endonuclease-sensitive sites was induced by UV in L5178Y, Q31, and human Raji cells. L5178Y cells irradiated with 10 J/m2 removed 18% of sensitive sites from DNA of detection, whereas Raji cells eliminated about 60% of the sites. These results during incubation for 24 h, and Q31 cells removed 3% of the sites, a fraction less than the limit indicate that mouse-lymphoma cells are capable of excision repair to a limited extend as compared with human cells and that mutant Q31 cells are essentially devoid of dimer excision. The newly synthesized DNA was of smaller size in UV-irradiated and unirradiated Q31 cells than that in the corresponding L5178Y cells, but the DNAs in both cell strains increased to comparable sizes after a 2-h chase.

References

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Citations

May 1, 1985·Somatic Cell and Molecular Genetics·P G McKennaV J McKelvey
Nov 1, 1988·Somatic Cell and Molecular Genetics·L H ThompsonS A Stewart
Sep 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·T HoriK Sato
May 1, 1988·The Journal of protozoology·D Ammermann
Dec 18, 2001·Environmental and Molecular Mutagenesis·R S TebbsL H Thompson
Sep 1, 1988·International Journal of Radiation Biology·J S Rubin
Jul 16, 1982·Biochemical and Biophysical Research Communications·D E LeeM W Lambert

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