DNA repair in microgravity: studies on bacteria and mammalian cells in the experiments REPAIR and KINETICS

Journal of Biotechnology
G HorneckC Schmitz

Abstract

The impact of microgravity on cellular repair processes was tested in the space experiments REPAIR and KINETICS, which were performed during the IML-2 mission in the Biorack of ESA: (a) survival of spores of Bacillus subtilis HA101 after UV-irradiation (up to 340 J m-2) in the experiment REPAIR; (b) in the experiment KINETICS the kinetics of DNA repair in three different test systems: rejoining of X-ray-induced DNA strand breaks (B1) in cells of Escherichia coli B/r (120 Gy) and (B2) in human fibroblasts (5 and 10 Gy) as well as (B3) induction of the SOS response after gamma-irradiation (300 Gy) of cells of Escherichia coli PQ37. Cells were irradiated prior to the space mission and were kept in a non-metabolic state (metabolically inactive spores of B. subtilis on membrane filters, frozen cells of E. coli and human fibroblasts) until incubation in orbit. Germination and growth of B. subtilis were initiated by humidification, E. coli and fibroblasts were thawed up and incubated at 37 degrees C for defined repair periods (up to 4.5 h), thereafter they were frozen again for laboratory analysis. Relevant controls were performed in-flight (1 x g reference centrifuge) and on ground (1 x g and 1.4 x g) The results show no significant ...Continue Reading

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Citations

Jan 13, 2000·Mutation Research·J Kiefer, H D Pross
Jan 22, 2002·Advances in Space Research : the Official Journal of the Committee on Space Research (COSPAR)·A TakahashiT Ohnishi
Jan 22, 2002·Advances in Space Research : the Official Journal of the Committee on Space Research (COSPAR)·T OhnishiS Nagaoka
Mar 4, 2010·Microbiology and Molecular Biology Reviews : MMBR·Gerda HorneckRocco L Mancinelli
Apr 29, 2005·Uchū Seibutsu Kagaku·Ken Ohnishi, Takeo Ohnishi
Mar 9, 2006·Radiation and Environmental Biophysics·Lorenzo Manti
Sep 24, 2009·Radiation Research·Birger DieriksPatrick Van Oostveldt
Jun 10, 2003·Journal of Radiation Research·Takeo OhnishiKen Ohnishi
Jun 10, 2003·Journal of Radiation Research·Akihisa TakahashiTakeo Ohnishi

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