PMID: 3762571Oct 1, 1986Paper

DNA-repair studies with sodium fluoride: comparative evaluation using density gradient ultracentrifugation and autoradiography

Mutation Research
J A SkareD B Cody

Abstract

Sodium fluoride (NaF) was assayed for the induction of DNA-repair synthesis in WI-38 human diploid fibroblasts and in primary cultures of rat hepatocytes. DNA-repair synthesis in non-replicating DNA was measured by ultracentrifugation of density-labeled DNA in CsCl gradients. When this method was used, NaF did not induce DNA-repair synthesis in either of these cell types. However, when NaF was assayed for induction of unscheduled DNA synthesis (UDS) in rat hepatocytes by autoradiography, an increased net nuclear grain count was observed. Because the autoradiographic results were not confirmed by density-gradient ultracentrifugation of hepatocyte DNA, which is a more definitive technique, it is doubtful whether the autoradiographic results actually represent DNA-repair synthesis. Modifications of the UDS/autoradiography protocol to include more extensive washing resulted in no UDS response. Published reports (Hellung-Larsen and Klenow, 1969; Srivastava et al., 1981) describe the formation of precipitable complexes of Mg2+, F-, and [3H]thymidine triphosphate which suggests that autoradiographic measurement of UDS may lead to artifacts when testing NaF unless extensive washing of the cultures is employed.

References

May 19, 1976·Biochimica Et Biophysica Acta·C A Smith, P C Hanawalt
Aug 1, 1973·The Journal of Cell Biology·R Hand, I Tamm
Aug 29, 1974·Biochimica Et Biophysica Acta·L N KappR R Klevecz
Jul 1, 1973·Experimental Cell Research·J R Gautschi, R M Kern
Mar 25, 1974·Journal of Molecular Biology·J R Gautschi
Oct 22, 1969·Biochimica Et Biophysica Acta·P Hellung-Larsen, H Klenow
Jan 1, 1972·Proceedings of the Society for Experimental Biology and Medicine·M Drescher, J W Suttie
Jul 16, 1981·Biochemical and Biophysical Research Communications·S K SrivastavaM J Modak
Jan 1, 1983·Environmental Mutagenesis·W J CoppingerE D Thompson

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Citations

Jun 1, 1988·Cell Biology and Toxicology·C C TongG M Williams
Jan 1, 1988·The Science of the Total Environment·G E Smith
Jul 1, 1989·Mutation Research·E D ThompsonD B Cody
Feb 1, 1990·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·K L SkareE D Thompson
Oct 1, 1996·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·G M WilliamsG S Allgood
Jan 23, 2003·International Journal of Toxicology·Monice Zondlo Fiume
Jan 1, 1993·Environmental and Molecular Mutagenesis·E ZeigerK L Witt
Jan 1, 1990·Critical Reviews in Oral Biology and Medicine : an Official Publication of the American Association of Oral Biologists·L S KaminskyM J Miller
Jan 1, 1991·Environmental and Molecular Mutagenesis·E D ThompsonD B Cody
Jan 1, 1989·Environmental and Molecular Mutagenesis·E D ThompsonR A LeBoeuf
Jan 1, 1988·Social Science & Medicine·G E Smith

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