PMID: 6980201May 1, 1982Paper

DNA repair synthesis in minimally stressed human lymphocytes

International Journal of Radiation Biology and Related Studies in Physics, Chemistry, and Medicine
B ForellA Norman

Abstract

We have estimated the rate of unscheduled DNA synthesis (UDS) in human lymphocytes from measurements of tritiated thymidine incorporation into double-stranded DNA (ds-DNA) during incubation of cells in vitro. Cells were not subjected to stresses except those associated with careful handling, or in certain experiments, mild heating or treatment with phytohaemagglutinin (PHA). Contribution of scheduled DNA synthesis (SDS) to incorporation was reduced by inhibiting replication and separating freshly replicated single-stranded DNA from repaired ds-DNA by chromatography. By increasing the incubation temperature, which decreases SDS and increases UDS, the residual contribution of scheduled DNA synthesis to thymidine incorporation into ds-DNA was estimated. Effects of increasing the number of cells in S-phase by phytohaemagglutinin were also investigated. Results suggest that: the rate of unscheduled DNA synthesis is about 500 +/- 100 thymidine molecules incorporated per cell per hour; a temperature-sensitive process, probably hydrolysis of DNA, contributes much of the damage repaired by UDS; background ionizing radiation contributes little to the damage; and damage caused by DNA hydrolysis is repaired much more efficiently than letha...Continue Reading

References

Jan 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·W A Deutsch, S Linn
Mar 1, 1979·Photochemistry and Photobiology·M S TangM H Patrick
Jul 1, 1979·International Journal of Radiation Oncology, Biology, Physics·L S Myers, E Kay
Jul 1, 1979·International Journal of Radiation Oncology, Biology, Physics·M M Elkind
Feb 1, 1972·Mutation Research·R B Painter, B R Young
Dec 4, 1973·Biochemistry·T Lindahl, O Karlström
Sep 12, 1972·Biochemistry·T Lindahl, B Nyberg
Oct 27, 1972·Biochimica Et Biophysica Acta·J R GautschiR B Painter
Jul 31, 1972·Biochimica Et Biophysica Acta·R E PyeritzC A Thomas
Dec 1, 1971·Mutation Research·W G Connor, A Norman
Nov 22, 1969·Nature·B DjordjevicM K Weill

❮ Previous
Next ❯

Citations

Sep 3, 1998·Mutation Research·M V FilatovL A Noskin

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.