DNA replication defect in the Escherichia coli cgtA(ts) mutant arising from reduced DnaA levels

Archives of Microbiology
Aleksandra SikoraGrzegorz Wegrzyn

Abstract

In Escherichia coli and other bacteria, the ribosome-associated CgtA GTP-binding protein plays a critical role in many basic cellular processes, including the control of DNA replication and/or segregation. However, the mechanism of this control is largely unknown. Here we report that ectopic expression of the dnaA gene partially restored both early growth in liquid medium and DNA synthesis defects of the cgtA(ts) mutant. Amounts of DnaA protein in the cgtA(ts) mutant incubated at elevated (42 degrees C) temperature were significantly lower relative to wild-type bacteria. Both level of dnaA mRNA and transcriptional activity of the dnaA promoter-lacZ fusion were decreased in the CgtA-deficient cells. The effects of ectopic expression of dnaA were specific as analogous expression of another gene coding for a replication regulator, seqA, had no significant changes in growth and DNA synthesis in the cgtA mutant. Thus, it appears that the DNA replication defect in this mutant is a consequence of reduced DnaA levels.

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Citations

Jul 10, 2007·Journal of Bacteriology·Mengxi JiangJanine R Maddock
May 6, 2008·Journal of Bacteriology·Sangita ShahRupak K Bhadra
Sep 3, 2011·Microbiology and Molecular Biology Reviews : MMBR·Natalie VerstraetenJan Michiels
Dec 23, 2008·Cardiovascular Research·Patrick LacolleyVeronique Regnault
Dec 20, 2015·FEMS Microbiology Reviews·Cheryl K Y LauHans J Vogel
Dec 1, 2009·Plasmid·Monika MaciagAgnieszka Szalewska-Pałasz
Jun 27, 2009·Molecular Microbiology·Nicole S PerskySusan T Lovett
Mar 30, 2013·Critical Reviews in Microbiology·Cyrielle KintJan Michiels
Jan 25, 2018·FEMS Microbiology Reviews·Liselot DewachterJan Michiels
Jul 14, 2017·Frontiers in Microbiology·Liselot DewachterJan Michiels
Dec 22, 2019·International Journal of Molecular Sciences·Liselot DewachterJan Michiels
Feb 2, 2021·Frontiers in Cellular and Infection Microbiology·Wolfgang EisenreichWerner Goebel
Aug 17, 2021·Molecular and Cellular Biology·Jaswinder Kaur, Kaustuv Datta

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