PMID: 1202023Dec 1, 1975Paper

DNA replication in mammalian cells. Altered patterns of initiation during inhibition of protein synthesis

The Journal of Cell Biology
R Hand

Abstract

The effects of inhibition of protein synthesis by the antibiotics cycloheximide and puromycin on the initiation of DNA replication in mouse L cells were studied. Cellular DNA was pulse labeled with [3H]thymidine of high, then of low specific activity and prepared for fiber autoradiography. Autoradiograms containing multiple (up to four) replication units were analyzed. In control cells, the proportion of replication units that initiated during a 10-min, high specific activity pulse was approximately equal to the proportion initiating immediately before the pulse. The addition of cycloheximide or puromycin at the start of the pulse inhibited the frequency of initiation in that there was a decrease by up to one-third of units initiating during the pulse relative to controls. Replication direction was also altered. Addition of the antibiotics 2 h before the pulse reduced the proportion of bidirectional units observed from 0.98 to 0.70. Antibiotic treatment for 2 h also decreased initiation synchrony in that the proportion of multiunit autoradiograms on which neighboring units showed similar replication patterns (indicating temporally coordinated initiation) was reduced by one-half. These observations indicate that inhibition of pr...Continue Reading

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Citations

Oct 1, 1992·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·J L Hamlin
Jul 1, 1976·Proceedings of the National Academy of Sciences of the United States of America·R L Seale
Dec 1, 1977·The Journal of Cell Biology·A S Weissfeld, H Rouse
Jun 1, 1980·The Journal of Cell Biology·M Zannis-HadjopoulosR Hand
Mar 9, 1992·FEBS Letters·D SchulteH Probst
May 1, 1986·International Journal of Radiation Biology and Related Studies in Physics, Chemistry, and Medicine·R B Painter
Jan 1, 1979·Journal of Cellular Physiology·W T MelvinH M Keir
Jul 1, 1979·Experimental Cell Research·A Richter, R Hand
Sep 25, 1977·Journal of Molecular Biology·E StimacJ A Huberman
Mar 1, 1988·Experimental Cell Research·V Gekeler, H Probst
Nov 1, 1980·Journal of Cellular Physiology·E EilenC Basilico
Nov 1, 1980·The American Journal of Anatomy·A Sato, S S Spicer

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