PMID: 19920550Apr 1, 1994Paper

DNA replication in the cat trabecular meshwork after laser trabeculoplasty in vivo

Journal of Glaucoma
S S BylsmaE M Van Buskirk

Abstract

The in vivo response to laser trabeculoplasty (LTP) was evaluated by measuring the incorporation of thymidine into DNA in the nuclei of trabecular meshwork cells. incorporation into DNA was analyzed by light microscopic autoradiography and by scintillation counting of trabecular extracts. Sixteen cats received argon LTP at a power setting of either 0.3 or 1.0 watt with the contralateral eye serving as a control; a 48-h exposure to HT began 1, 5, or 12 days later. The level of HT incorporation into DNA for LTP-treated eyes was significantly higher than controls for the earliest labeling period, but not at the later time points. This pattern was observed for both 0.3-and 1.0-watt treatments. In a second experiment, LTP was performed on six animals; a power setting of 0.3 watt was used in the left eye, and a power setting of 1 watt was used in the right eye. All 12 eyes were radiolabeled for 48 h with HT beginning 1 day after LTP. A small but significant difference in incorporation levels was found between these two power settings. Trabecular cell division may play a role in the therapeutic efficacy of LTP in glaucoma patients.

Citations

Nov 4, 2008·Experimental Eye Research·Abbot F Clark, Robert J Wordinger
Dec 18, 2013·Clinical & Experimental Ophthalmology·Dov B KaganCindy M L Hutnik
Feb 16, 2007·Current Opinion in Ophthalmology·Joshua D Stein, Pratap Challa

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.