DNA replication is completed in Saccharomyces cerevisiae cells that lack functional Cdc14, a dual-specificity protein phosphatase

Molecular & General Genetics : MGG
P J FitzpatrickL H Johnston

Abstract

The Cdc14 protein encodes a dual-specificity protein phosphatase which functions in late mitosis, and considerable genetic evidence suggests a role in DNA replication. We find that cdc14 mutants arrested in late mitosis maintain persistent levels of mitotic kinase activity, suggesting that Cdc14 controls inactivation of this kinase. Overexpression of Sicl, a cyclin-dependent protein kinase inhibitor, is able to suppress telophase mutants such as dbf2, cdc5 and cdc15, but not cdc14. It does, however, force cdc14-arrested cells into the next cell cycle, in which an apparently normal S phase occurs as judged by FACS and pulsed-field gel electrophoretic analysis. Furthermore, in a promoter shut-off experiment, cells lacking Cdc14 appear to carry out a normal S phase. Thus Cdc14 functions mainly in late mitosis and it has no essential role in S phase.

Citations

Nov 30, 2010·Bioprocess and Biosystems Engineering·Kazuhiro MaedaHiroyuki Kurata
Aug 12, 2009·Proceedings of the National Academy of Sciences of the United States of America·Stanimir DulevAlexander Strunnikov
Jun 14, 2006·The Journal of Cell Biology·Felix MachínLuis Aragón
Apr 4, 2006·Biophysical Journal·Attila Csikász-NagyJohn J Tyson
Apr 12, 2012·Journal of the Royal Society, Interface·Ian D LoramPeter J Gawthrop

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