DNA-RNA hybridization

Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences
J O BishopS M Perlman


Interest in nucleic acid hybridization stems mainly from its great power as a tool in biological research. It is used in several quite distinct ways. Because of the high degree of specificity that they show, hybridization techniques can be used to measure the amount of one specific sequence within a very heterogeneous mixture of sequences. Measurements of 1/10(6)-10(7) have been recorded. In extension of this, various properties of a specific sequence can often be studied. Secondly, because the kinetics of nucleic acid hybridization are quite well understood, it can be used to characterize both a pure sequence and a very complex mixture of sequences, like the genome of a vertebrate. Thirdly, again because of its specificity, it can be used to measure homologies between different populations of nucleic acids. Lastly, in conjunction with other techniques, it can be used as a basis for the fractionation of nucleic acid populations and the purification of specific sequences. Specific examples of these applications are given, with special reference to the organization of the genome in higher eukaryotes.


Jun 1, 1979·Biochemical Genetics·M Izquierdo, J O Bishop
Dec 1, 1979·Journal of Molecular Evolution·E Zuckerkandl
Aug 1, 1978·Developmental Biology·J F JacksonD I de Pomerai
Sep 1, 1982·Developmental Biology·M T Imaizumi-ScherrerK Scherrer
Oct 1, 1984·Developmental Biology·N PerrimonA P Mahowald
Sep 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·S M PerlmanM M Rosbash
Aug 24, 1979·Nucleic Acids Research·J R JenkinsP H Butterworth
Jun 1, 1978·Nature·A Garcia-Bellido, P Ripoll
Jan 1, 1980·Annals of the New York Academy of Sciences·T A RapoportS Rosenthal
Nov 15, 1978·European Journal of Biochemistry·S J HigginsD G Herries

Related Concepts

DNA Sequence
DNA, Double-Stranded
Drosophila melanogaster
Genomic Hybridization
Adenine Polynucleotides

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