DNA sensor for o-dianisidine

Bioelectrochemistry
J JasnowskaM Filipiak

Abstract

o-Dianisidine (3,3'-dimethoxybenzidine) is applied in the production of some dyes and also used in analytical tests. However, this compound is anticipated to be a human carcinogen. An analytical strategy utilizing square wave voltammetry for the determination of o-dianisidine is presented. An electrochemical system was consisted of three electrodes: carbon paste working electrode, platinum wire counter electrode and silver-silver chloride (Ag/AgCl) reference electrode. However, square wave voltammograms of direct measurements of o-dianisidine were found to be hardly reproducible, exhibiting few peaks due to some labile short-lived intermediates with the only exception of a quite stable peak at +0.7 V vs. Ag/AgCl. Quantitative determination of o-dianisidine gave satisfactory results only when the carbon paste working electrode was replaced by deoxyribonucleic acids (DNA) electrode obtained by immobilization of double-stranded (ds) DNA on carbon electrode. Square wave voltammogram of DNA showed two peaks attributed to adenine and guanine and the latter was used as analytical signal. After interaction with o-dianisidine, guanine oxidation peak was reduced to the extent related to the concentration of the analyte. Initial reduction...Continue Reading

References

Aug 10, 2000·Nucleic Acids Research·J Wang
Feb 24, 2001·Fresenius' Journal of Analytical Chemistry·M MasciniG Marrazza
Oct 29, 2002·Bioelectrochemistry·F LucarelliM Mascini

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Citations

Sep 2, 2011·Applied Biochemistry and Biotechnology·Bahar DindarFatih Abasıyanık
Sep 19, 2006·Bioelectrochemistry·I StempkowskaM Filipiak
Jul 6, 2013·Materials Science & Engineering. C, Materials for Biological Applications·Leila MirmoghtadaieParviz Norouzi
Sep 13, 2005·Artificial Cells, Blood Substitutes, and Immobilization Biotechnology·Emine KarakuşKiliç Esma
May 6, 2020·Biomolecules·Oskar SzczepaniakPiotr Szulc
Oct 22, 2021·Biotechnology and Bioengineering·Anjum Hamid RatherFaheem A Sheikh

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