PMID: 2498292Jun 1, 1989Paper

DNA sequence and regulation of the gene (cbpA) encoding the 42-kilodalton cytoplasmic membrane carotenoprotein of the cyanobacterium Synechococcus sp. strain PCC 7942

Journal of Bacteriology
K J ReddyL A Sherman

Abstract

The gene (cbpA) coding for a carotenoid-binding protein of the cyanobacterium Synechococcus sp. strain PCC 7942 (Anacystis nidulans R2) has been cloned and sequenced. A polyclonal antibody against the protein was used to identify immunoreactive clones from a lambda gt11 expression library of Synechococcus strain PCC 7942. The initial positive clone (lambda gtAN42) contained a 0.9-kilobase (kb) chromosomal fragment, which was used to detect a larger chromosomal fragment from a lambda EMBL3 library. The lambda EMBL3 recombinant, lambda EM109, contained an 18-kb portion of the Synechococcus strain PCC 7942 chromosome. The open reading frame of cbpA encoded 450 amino acids which give rise to a protein of 49,113 daltons. The hydrophobicity plot indicates that the protein may have a 49-residue signal sequence which is cleaved to yield a mature protein of 43,709 daltons. The protein has been localized in the cytoplasmic membrane by biochemical procedures as well as by electron microscopic immunocytochemistry. Northern (RNA) blot analysis indicates that transcription of cbpA is tightly regulated by DNA topology, light intensity, and iron concentration. Transcription is greatly induced by growth under high light intensities and represse...Continue Reading

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Citations

Oct 12, 2005·Journal of Experimental Botany·Murray R BadgerFiona J Woodger
Jul 5, 2017·The New Phytologist·Cheryl A KerfeldMaria Agustina Dominguez-Martin
Jan 20, 1999·Proceedings of the National Academy of Sciences of the United States of America·S ReumannK Keegstra
Jan 14, 2020·Biochimica Et Biophysica Acta. Molecular and Cell Biology of Lipids·Ranganathan ArunkumarPaul S Bernstein
Jan 8, 1999·Biochimica Et Biophysica Acta·B Fernández-GonzálezA Vioque
Apr 1, 1994·Journal of Bacteriology·M M Mackle, B A Zilinskas
May 20, 2015·ACS Synthetic Biology·C Josh RameyNanette R Boyle

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