PMID: 7022127Jan 1, 1981

DNA sequence of a plasmid-encoded dihydrofolate reductase

Molecular & General Genetics : MGG
G SwiftF Heffron

Abstract

The sequence of the methotrexate-resistant dihydrofolate reductase (DHFR) gene borne by the plasmid R-388 was determined. The gene was subcloned and mapped by an in vitro mutagenesis method involving insertion of synthetic oligonucleotide decamers encoding the BamHI recognition site. Sites of insertion that destroyed the methotrexate resistance fell in two regions separated by 300 bp within a 1.2 kb fragment. One of these regions encodes a 78 amino acid polypeptide homologous to another drug-resistant DHFR. The second region essential for DHFR expression appears to be the promoter of the DHFR gene.

References

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Dec 2, 1975·Biochemistry·D BaccanariJ J Burchall
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Jun 4, 1971·Biochemical and Biophysical Research Communications·J S Erickson, C K Mathews

Related Concepts

Alkalescens-Dispar Group
SYN8DHFRS
DHFR
Promoter
Deoxyribonuclease BamHI
Oligonucleotides
Tetrahydrofolate Dehydrogenase
Plasmids
Mutagenesis Procedure
Cistron

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