PMID: 8607286Nov 1, 1995Paper

DNA sequence of the deletion/insertion in early region 3 of Ad5 dl309

Virus Research
A J BettF L Graham

Abstract

dl309 is an adenovirus type 5 (Ad5) mutant that has been extensively utilized for construction of Ad5 mutants in early region 1 (E1), in developing vectors for use as viral vaccines, and in development of gene transfer vectors for gene therapy. Ad5 dl309 has been useful for vector construction because of its altered XbaI restriction pattern and lends itself to a variety of strategies for rescuing inserts or mutations into E1. It contains only one XbaI site at 3.7 map units (m.u.) as compared to wt Ad5 which contains 4(3.7, 29.5, 79.5, and 84.8 m.u.). The loss of the sites at 29.5 and 79.5 m.u. is due to deletions of a few bp but the loss of the site at 84.8 m.u. was the result of a deletion from approximately 83 to 85 m.u. and substitution with a fragment of foreign DNA. Because of the widespread use of dl309 and derivatives of this mutant in the construction of Ad5-based vectors and the need to have precise genetic information on the sequences present in vectors to be used as vaccines and in gene therapy, we have sequenced the alterations in dl309 which affect the XbaI sites at 79.5 and 84.8 m.u. and have determined which E3 proteins are expressed by this virus. The deletion that removes the XbaI site at 84.8 m.u. extends from...Continue Reading

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Citations

Jan 20, 2006·Archives of Virology·C Möritz, M Dobbelstein
Jan 19, 2000·Drug Discovery Today·I Wang, I Huang
May 3, 2001·Journal of Virology·A FrançoisP Langlois
Dec 24, 2003·International Reviews of Immunology·Drew L LichtensteinWilliam S M Wold
Jul 4, 2002·Molecular Therapy : the Journal of the American Society of Gene Therapy·Cheryl A CarlsonAndré Lieber
Jan 9, 2008·Molecular Therapy : the Journal of the American Society of Gene Therapy·E Antonio ChioccaDavid Kirn
Jan 25, 2019·PloS One·Leandro CrisostomoPeter Pelka
Nov 3, 2005·Evolution; International Journal of Organic Evolution·W Bryan Jennings, Scott V Edwards
Oct 3, 1999·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·K Ghosh, H P Ghosh

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