PMID: 2120198Oct 1, 1990Paper

DNA sequence of the purC gene encoding 5'-phosphoribosyl-5-aminoimidazole-4-N-succinocarboxamide synthetase and organization of the dapA-purC region of Escherichia coli K-12

Journal of Bacteriology
A A TiedemanJ M Smith

Abstract

5'-Phosphoribosyl-5-aminoimidazole-4-N-succinocarboxamide synthetase (EC 6.3.2.6), encoded by the purC gene of Escherichia coli K-12, catalyzes the synthesis of 5'-phosphoribosyl-5-aminoimidazole-4-N-succinocarboxamide from 5'-phosphoribosyl-5-aminoimidazole-4-carboxylic acid. The mature protein, as deduced from the purC structural gene sequence, contains 237 amino acids and has a calculated Mr of 26,998. The control region of the purC gene was identified by primer extension mapping of the 5' end of the purC mRNA. The purC control region contains a binding site for and is regulated by the purine repressor, the product of the purR gene. An unusual feature of the 5' untranslated region of the purC mRNA is the presence of a repetitive extragenic palindrome sequence normally found in intercistronic or 3' untranslated regions. The DNA sequence was extended 1.281 kilobases upstream of the purC structural gene and overlapped with the previously determined dapA sequence. Termination of transcription from the dapA-purC intercistronic region may occur within the -35 region of the purC control region. The purC gene has been positioned on the E. coli restriction map and is transcribed in a counterclockwise direction.

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Citations

May 16, 1998·The Journal of Biological Chemistry·H XuK S Matthews
May 17, 2011·Nucleic Acids Research·Byung-Kwan ChoBernhard Ø Palsson
Aug 1, 1992·Journal of Bacteriology·J BouvierP Stragier
Oct 1, 1993·Journal of Bacteriology·F M Hui, D A Morrison
Sep 1, 2008·EcoSal Plus·Kaj Frank JensenMartin WillemoËs
Sep 8, 1998·Microbiology and Molecular Biology Reviews : MMBR·M K Berlyn
Dec 1, 1993·Microbiological Reviews·M Riley

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