PMID: 6990409Mar 1, 1980Paper

DNA sequence of the Serratia marcescens lipoprotein gene

Proceedings of the National Academy of Sciences of the United States of America
K Nakamura, M Inouye

Abstract

The Serratia marcescens gene for the outer membrane lipoprotein (lpp) was cloned in lambda phage vector Charon 14. The recombinant phage was very unstable, and the lpp gene with a 300-base-pair deletion at the transcription termination site was further cloned in pBR322. The DNA sequence of 834 base pairs encompassing the lpp gene was determined and compared with that of the Escherichia coli lpp gene. The sequence comparisons exhibit several unique features. (i) The promoter region is highly conserved (84% homology) and has an extremely high A+T content (78%) as in E. coli (80%). (ii) The 5' nontranslated region of the lipoprotein mRNA is also highly conserved (95% homology). (iii) In the DNA sequence corresponding to the signal peptide of this secretory protein, there are three drastic changes, including addition of one base pair and deletion of four base pairs in S. marcescens as compared to E. coli. The resultant alterations in the amino acid sequence, however, do not change the basic properties of the signal peptide, which are assumed to be essential for its function in the secretory mechanism. (iv) The DNA sequence from the amino terminus to the 51st residue of the mature lipoprotein is highly conserved (95% homology) and t...Continue Reading

References

Dec 20, 1979·Nucleic Acids Research·J P GergenP C Wensink
Feb 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·A M Maxam, W Gilbert
Mar 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·S InouyeM Inouye
May 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·R M PirtleM Inouye
Jan 1, 1978·Annual Review of Biochemistry·J M DiRienzoM Inouye
Jan 1, 1978·Annual Review of Biochemistry·S Adhya, M Gottesman
Dec 14, 1978·Nature·G F Miozzari, C Yanofsky
Jan 1, 1976·Annual Review of Microbiology·K E Sanderson
Mar 1, 1975·Proceedings of the National Academy of Sciences of the United States of America·T ManiatisD G Kleid
Jul 15, 1974·Journal of Molecular Biology·P N BorerO C Uhlenbeck
Nov 14, 1973·Nature: New Biology·I TinocoJ Bralla
Aug 1, 1972·Proceedings of the National Academy of Sciences of the United States of America·S N CohenL Hsu

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Citations

Jan 1, 1980·Molecular & General Genetics : MGG·P OstapchukM Riley
Jun 1, 1990·Journal of Bioenergetics and Biomembranes·S Hayashi, H C Wu
Jan 1, 1983·The International Journal of Biochemistry·I Suominen, P Mäntsälä
Jan 1, 1982·Biophysical Journal·H C WuC Z Giam
Dec 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·T MiyataT Nishida
Feb 1, 1986·Journal of Biomolecular Structure & Dynamics·V BrendelE N Trifonov
Jul 11, 1984·Nucleic Acids Research·M E Watson
Jan 10, 1981·Nucleic Acids Research·R GranthamR Mercier
Dec 21, 1981·Nucleic Acids Research·C YanofskyA M Wu
Apr 13, 2005·The Journal of Hospital Infection·A DubouixN Marty
Aug 15, 2018·FEMS Microbiology Letters·Abir T Asmar, Jean-François Collet
Jan 1, 1980·Annals of the New York Academy of Sciences·M InouyeI Pirtle
Mar 28, 1998·Microbial Pathogenesis·I HironoT Aoki
Feb 1, 1990·Infection and Immunity·M A SwancuttM V Norgard
Nov 1, 1988·Infection and Immunity·K Poole, V Braun
Jul 1, 1988·Journal of Bacteriology·K PooleV Braun

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