DNA sequence recognition by an isopropyl substituted thiazole polyamide

Nucleic Acids Research
Peter L JamesK R Fox

Abstract

We have used DNA footprinting and fluorescence melting experiments to study the sequence-specific binding of a novel minor groove binding ligand (thiazotropsin A), containing an isopropyl substituted thiazole polyamide, to DNA. In one fragment, which contains every tetranucleotide sequence, sub-micromolar concentrations of the ligand generate a single footprint at the sequence ACTAGT. This sequence preference is confirmed in melting experiments with fluorescently labelled oligonucleotides. Experiments with DNA fragments that contain variants of this sequence suggest that the ligand also binds, with slightly lower affinity, to sequences of the type XCYRGZ, where X is any base except C, and Z is any base except G.

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Citations

Nov 26, 2008·Chemphyschem : a European Journal of Chemical Physics and Physical Chemistry·Gudrun M SpitzerKlaus R Liedl
Apr 29, 2006·Bioorganic & Medicinal Chemistry Letters·Andrew J HampshireKeith R Fox
Sep 13, 2011·European Journal of Medicinal Chemistry·Abedawn I KhalafColin J Suckling
Dec 2, 2017·Nucleic Acids Research·Giacomo PadroniGlenn A Burley
Jul 7, 2018·Beilstein Journal of Organic Chemistry·Sayantan BhaduriDev P Arya
Mar 15, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Ananya PaulW David Wilson
Mar 12, 2009·Journal of Chemical Information and Modeling·Gudrun M SpitzerKlaus R Liedl
Nov 11, 2010·ACS Medicinal Chemistry Letters·Kitiyaporn WittayanarakulSimon P Mackay
Nov 29, 2005·Journal of Chemical Information and Modeling·Nahoum G AnthonySimon P Mackay
Oct 27, 2007·Journal of Medicinal Chemistry·Nahoum G AnthonyRoger D Waigh

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