PMID: 9546195Apr 18, 1998Paper

DNA sequence similarity between California isolates of Cryptosporidium parvum

Applied and Environmental Microbiology
G Pereira M dasR B Lefebvre

Abstract

We evaluated whether nucleic acid amplification with primers specific for Cryptosporidium parvum followed by automated DNA sequence analysis of the PCR amplicons could differentiate between California isolates of C. parvum obtained from livestock, humans, and feral pigs. Almost complete sequence identity existed among the livestock isolates and between the livestock and human isolates. DNA sequences from feral pig isolates differed from those from livestock and humans by 1.0 to 1.2%. The reference sequence obtained by Laxer et al. (M. A. Laxer, B. K. Timblin, and R. J. Patel, Am. J. Trop. Med. Hyg. 45:688-694, 1991.) differed from California isolates of C. parvum by 1.8 to 3.2%. These data suggest that DNA sequence analysis of the amplicon of Laxer et al. does not allow for differentiation between various strains of C. parvum or that our collection of isolates obtained from various hosts from across California was limited to one strain of C. parvum.

References

Dec 1, 1991·The American Journal of Tropical Medicine and Hygiene·M A LaxerR J Patel
Dec 1, 1989·Epidemiology and Infection·H V SmithG I Forbes
May 25, 1989·The New England Journal of Medicine·E B HayesM L Cummings
Jul 21, 1994·The New England Journal of Medicine·W R Mac KenzieJ B Rose
Feb 1, 1996·Applied and Environmental Microbiology·X LengR D Oberst
Jul 1, 1996·Journal of Clinical Microbiology·A B BalatbatJ Silva

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Citations

Feb 24, 1999·Australian Veterinary Journal·U M MorganR C Thompson
Jul 4, 2003·Applied and Environmental Microbiology·U M RyanR C A Thompson
Sep 11, 2004·The Journal of Parasitology·U M RyanL Xiao
Jan 5, 2000·International Journal for Parasitology·U M MorganR C Thompson
Dec 13, 2000·International Journal for Parasitology·R FayerS J Upton

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