DNA sequence variation as a clue to the phylogenesis of orthopoxviruses

The Journal of General Virology
N J Douglas, K R Dumbell

Abstract

We have sequenced DNA equivalent to the E5R ORF of Copenhagen vaccinia virus from an additional strain of vaccinia and from cowpox (three strains), camelpox (two strains), taterapox and ectromelia viruses. None of these showed the disruptions previously reported in the equivalent region of monkeypox virus. We also constructed a viable recombinant of vaccinia virus strain Dairen in which the E5R sequence was disrupted by a 436 bp deletion and substitution of the E. coli gpt gene. Quantitative analysis of the sequences, including available sequences from monkeypox, variola and vaccinia viruses revealed four main groupings, namely cowpox, ectromelia, monkeypox and a cluster which includes variola, camelpox, taterapox and vaccinia viruses. It was noted that, at over 75 % of the positions which differentiated species. all species but one had a common nucleotide. Although the analysis covers one single gene only, the results accord with what is known of the biology of the viruses.

Citations

Dec 11, 2003·American Journal of Physical Anthropology·Linda M Van Blerkom
Dec 6, 2001·FEBS Letters·S N ShchelkunovL S Sandakhchiev
Jul 1, 1997·Zentralblatt für Veterinärmedizin. Reihe B. Journal of veterinary medicine. Series B·L Haas
Oct 31, 1998·Infection·P D Ellner
Mar 22, 2002·The Journal of General Virology·Caroline Gubser, Geoffrey L Smith
Nov 24, 1999·Virology·D O WillerD H Evans
Apr 9, 2008·Pathologie-biologie·C Chastel

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