PMID: 2105306Feb 1, 1990Paper

DNA sequences and characterization of four early genes of the tryptophan pathway in Pseudomonas aeruginosa

Journal of Bacteriology
D W EssarI P Crawford

Abstract

Two pairs of related but easily distinguishable genes for the two subunits of anthranilate synthase have been identified in Pseudomonas aeruginosa. These were cloned, sequenced, inactivated in vitro by insertion of an antibiotic resistance cassette, and returned to the P. aeruginosa chromosome, replacing the wild-type gene. Gene replacement implicated only one of the pairs in tryptophan biosynthesis. This report describes the cloning and sequencing of the tryptophan-related gene pair, designated trpE and trpG, and presents experiments implicating their gene products in tryptophan production. DNA sequence analysis as well as growth and enzyme assays of insertionally inactivated strains indicated that trpG is the first gene in a three-gene operon that also includes trpD and trpC. Complementation of Trp auxotrophs by R-prime plasmids (T. Shinomiya, S. Shiga, and M. Kageyama, Mol. Gen. Genet., 189:382-389, 1983) has shown that a large cluster of pyocin R2 genes is flanked at one end by trpE and the other end by trpDC; the physical map that was obtained shows the distance between trpE and trpDC to be about 25 kilobases. Our restriction map of the trpE and trpGDC regions agrees with data presented by Shinomiya et al.

References

Mar 1, 1979·Microbiological Reviews·B W HollowayA F Morgan
Sep 1, 1979·Microbiological Reviews·T Leisinger, R Margraff
Mar 1, 1978·FEBS Letters·F Sanger, A R Coulson
Jun 1, 1975·Bacteriological Reviews·I P Crawford
Apr 1, 1989·Biochimie·I P Crawford, L Eberly
Jan 1, 1989·Annual Review of Microbiology·I P Crawford
Jan 1, 1985·Gene·B D Gambill, A O Summers
Apr 1, 1974·Proceedings of the National Academy of Sciences of the United States of America·J Shine, L Dalgarno
Mar 1, 1973·Molecular & General Genetics : MGG·D H CalhounR A Jensen
Oct 14, 1970·Journal of Molecular Biology·M Mandel, A Higa
May 1, 1971·Journal of Bacteriology·R Maurer, I P Crawford
Jan 1, 1980·Methods in Enzymology·A M Maxam, W Gilbert
Jan 1, 1981·Cold Spring Harbor Symposia on Quantitative Biology·D E BergT D Tlsty
Aug 15, 1983·Journal of Molecular Biology·J B Kaplan, B P Nichols
Jan 11, 1984·Nucleic Acids Research·L M LagriminiJ E Donelson

❮ Previous
Next ❯

Citations

Sep 27, 2001·Proceedings of the National Academy of Sciences of the United States of America·M W CalfeeE C Pesci
Aug 16, 2008·Molecular BioSystems·Jean-Frédéric Dubern, Stephen P Diggle
Jan 1, 2009·Microbial Biotechnology·M Antonia Molina-HenaresEstrella Duque
Aug 27, 2010·FEMS Microbiology Reviews·Stephan HeebMiguel Cámara
May 26, 2010·Proceedings of the National Academy of Sciences of the United States of America·Sudha Chugani, Everett Peter Greenberg
Jan 15, 1994·FEMS Microbiology Letters·W Zimmer, B Hundeshagen
Oct 5, 2017·Scientific Reports·Megan G LloydChristopher T Nomura
Mar 31, 2019·Chembiochem : a European Journal of Chemical Biology·Bethany K OkadaMohammad R Seyedsayamdost
Jan 8, 2015·The Journal of Microbiology·Ehsan SepahiAbdolreza Bagheri
Nov 29, 2007·Cellular Microbiology·Laeticia AlibaudPierre Cosson
Jun 24, 2020·Antibiotics·Christine PourcelLibera Latino
Nov 12, 2002·Journal of Bacteriology·Larry A GallagherColin Manoil
Sep 4, 2004·Applied and Environmental Microbiology·Korneel RabaeyWilly Verstraete
Mar 6, 2007·Journal of Bacteriology·John M Farrow, Everett C Pesci
May 8, 2021·Frontiers in Microbiology·Nanami SakataYasuhiro Ishiga
Oct 1, 1992·Journal of Bacteriology·J BardowskiA Chopin
Aug 1, 1992·Journal of Bacteriology·M Becker-RudzikB L Marrs
Jun 1, 1991·Journal of Bacteriology·C Y HanC S Harwood
Feb 18, 2006·International Journal of Medical Microbiology : IJMM·Stephen P DiggleMiguel Cámara
Aug 13, 2010·Chemical Reviews·Holly Huse, Marvin Whiteley

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.