DNA sequencing by mass spectrometry

Journal of Mass Spectrometry : JMS
K K Murray

Abstract

Mass spectrometry has the potential to replace gel electrophoresis for fast DNA sequencing. In this tutorial paper, it is discussed how far mass spectrometry of DNA has come since the advent of electrospray ionization (ESI) and matrix-assisted laser desorption/ionization (MALDI) and how much remains to be done before mass spectrometry will be a useful tool for DNA sequencing. A brief description of MALDI and ESI mass spectrometric analysis of DNA is presented along with the different strategies for DNA sequencing using mass spectrometry. These include mass spectrometric analysis to replace gel separation in Sanger sequencing, enzymatic ladder sequencing and sequencing by gas-phase fragmentation. The future role of mass spectrometry in DNA sequencing in the Human Genome Project and beyond is also discussed.

Citations

Mar 11, 2011·Bioanalysis·William D van Dongen, Wilfried M A Niessen
Jul 25, 2000·Nucleic Acids Research·M BergerF E Romesberg
Feb 28, 2013·Chemical Reviews·Natalia TretyakovaSrikanth Kotapati
Sep 27, 2003·Expert Review of Molecular Diagnostics·Erwin SablonFabien Zoulim
Sep 7, 2001·Rapid Communications in Mass Spectrometry : RCM·J Ni, K Chan
Sep 25, 2004·Mass Spectrometry Reviews·Helen J CooperAlan G Marshall
Sep 30, 2003·Rapid Communications in Mass Spectrometry : RCM·Michael Mormann, Jasna Peter-Katalinić
Aug 22, 2006·Journal of Mass Spectrometry : JMS·Jörg Tost, Ivo Glynne Gut
Sep 17, 2005·Clinica Chimica Acta; International Journal of Clinical Chemistry·Afshin AhmadianSophia Hober
Jan 21, 2011·Proceedings of the National Academy of Sciences of the United States of America·Qing WangBert Vogelstein
Nov 5, 1998·Bioorganic & Medicinal Chemistry·D R OwensG Siuzdak
Nov 10, 2009·Journal of the American Society for Mass Spectrometry·Daniele Fabris
May 8, 2000·Journal of Mass Spectrometry : JMS·K M Downard
Apr 23, 2008·Current Protocols in Nucleic Acid Chemistry·C W Chou, P A Limbach
Mar 30, 2004·Journal of the American Society for Mass Spectrometry·Herbert OberacherChristian G Huber
Apr 12, 2002·Journal of Mass Spectrometry : JMS·John M KoomenDavid H Russell
Apr 12, 2002·Mass Spectrometry Reviews·C G Huber, H Oberacher
May 29, 2003·Rapid Communications in Mass Spectrometry : RCM·Yun XiangMitsuo Takayama
Apr 1, 2003·Mass Spectrometry Reviews·Jörg Tost, Ivo G Gut
Nov 24, 1999·Rapid Communications in Mass Spectrometry : RCM·H LinC H Becker
Apr 5, 2019·Journal of the American Society for Mass Spectrometry·Yang Liu, František Tureček
May 11, 2018·Journal of Mass Spectrometry : JMS·Yasemin Ucal, Aysel Ozpinar
Oct 19, 2017·Nature Communications·Abdelaziz Al OuahabiJean-François Lutz
Aug 21, 2001·Chemical Research in Toxicology·N TretyakovaS R Tannenbaum
Mar 21, 1998·Current Opinion in Biotechnology·P F Crain, J A McCloskey
Nov 27, 2002·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Jan Havlis, Martin Trbusek

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