Dec 1, 1977

DNA sequencing with chain-terminating inhibitors

Proceedings of the National Academy of Sciences of the United States of America
F SangerA R Coulson


A new method for determining nucleotide sequences in DNA is described. It is similar to the "plus and minus" method [Sanger, F. & Coulson, A. R. (1975) J. Mol. Biol. 94, 441-448] but makes use of the 2',3'-dideoxy and arabinonucleoside analogues of the normal deoxynucleoside triphosphates, which act as specific chain-terminating inhibitors of DNA polymerase. The technique has been applied to the DNA of bacteriophage varphiX174 and is more rapid and more accurate than either the plus or the minus method.

Mentioned in this Paper

DNA, Viral
DNA Polymerase I
Sequence Determinations, DNA
Base Sequence
DNA Restriction Enzymes
DNA-Directed DNA Polymerase

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