DNA strand breaks, acute phase response and inflammation following pulmonary exposure by instillation to the diesel exhaust particle NIST1650b in mice

Mutagenesis
Zdenka O KyjovskaUlla Vogel

Abstract

We investigated the inflammatory response, acute phase response and genotoxic effect of diesel exhaust particles (DEPs, NIST1650b) following a single intratracheal instillation. C57BL/6J BomTac mice received 18, 54 or 162 µg/mouse and were killed 1, 3 and 28 days post-exposure. Vehicle controls and the benchmark particle carbon black (CB, Printex 90; 162 µg/mouse) were tested alongside for comparison. The cellular composition and protein concentration were determined in bronchoalveolar lavage (BAL) fluid as markers for an inflammatory response. Pulmonary and systemic genotoxicity was analysed by the alkaline comet assay as DNA strand breaks in BAL cells, lung and liver tissue. The pulmonary acute phase response was analysed by Saa3 mRNA levels by real-time quantitative polymerase chain reaction. Instillation of DEP induced a strong neutrophil influx 1 and 3 days, but not 28 days post-exposure. Saa3 mRNA levels were increased at all time point for the highest dose and 28 days post-exposure for the middle dose. DEP increased levels of DNA strand breaks in lung tissue for all doses 1 day post-exposure and after 28 days for mid- and high-dose groups. Pulmonary exposure to DEP induced transient inflammation but long-lasting pulmonar...Continue Reading

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Citations

Aug 12, 2015·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Nicklas Raun JacobsenPeter Møller
Jan 20, 2018·American Journal of Physiology. Endocrinology and Metabolism·Minjie ChenZhekang Ying
Feb 2, 2018·Particle and Fibre Toxicology·Astrid SkovmandSandra Goericke-Pesch
Sep 12, 2018·Particle and Fibre Toxicology·Masakazu UmezawaKarin S Hougaard
Jun 12, 2019·Particle and Fibre Toxicology·Katja Maria BendtsenUlla Vogel
Jun 12, 2019·Particle and Fibre Toxicology·Maria Helena Guerra AndersenUlla Vogel
Feb 8, 2021·Environmental Health : a Global Access Science Source·Katja M BendtsenUlla Vogel
Feb 28, 2021·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Daniel Vest ChristophersenNicklas Raun Jacobsen
Aug 10, 2021·Environmental and Molecular Mutagenesis·Zhiying JiMatthew J LeBaron

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