PMID: 9539205Apr 16, 1998Paper

DNA strand breaks in ejaculated human spermatozoa: comparison of susceptibility to the nick translation and terminal transferase assays

The Histochemical Journal
G C ManicardiD Sakkas

Abstract

The nick translation and terminal transferase assays have been compared to test their relative efficiency in detecting DNA breakage in ejaculated human spermatozoa. The results have been correlated with the percentage of chromomycin A3 positive sperm, a fluorochrome that is indicative of the protamination state of sperm. Examination of the ejaculated sperm of 30 subjects revealed that the percentage of positivity to the nick translation and terminal transferase assays did not differ, even when using different fixatives. It is concluded that the inability of the two assays to distinguish the type of DNA damage, as is possible in somatic nuclei, is most probably linked to the unique nature of sperm chromatin. It is proposed that the presence of the damaged DNA may be the remnants of an imperfect spermiogenesis, probably related to an inadequate protamine deposition. This is supported by the strong correlation between the presence of DNA damage and underprotamination as evidenced by chromomycin A3.

Citations

Mar 22, 2002·Biology of Reproduction·Denny SakkasDavide Bizzaro
Nov 14, 2003·Journal of Clinical Laboratory Analysis·Fernando Tadeu Andrade-Rocha
Dec 24, 2005·Asian Journal of Andrology·J ErenpreissA Giwercman
Mar 7, 2018·Environmental Science and Pollution Research International·Ammar OumaimaMehdi Meriem
Sep 1, 2007·Reproductive Biology and Endocrinology : RB&E·Roxani AngelopoulouPavlos Msaouel
Jun 10, 2011·International Journal of Andrology·S Oehninger
Dec 4, 2012·International Journal of Molecular Sciences·Clara González-MarínRosa Roy
Apr 6, 2004·Journal of Andrology·Alexis M CodringtonBernard Robaire
Oct 16, 1999·The Journal of Clinical Endocrinology and Metabolism·D M De Kretser, H W Baker
Nov 24, 2001·Human Fertility : Journal of the British Fertility Society·G M Hartshorne
Mar 10, 2001·Molecular Reproduction and Development·N CaronG Boissonneault
Aug 20, 2010·Systems Biology in Reproductive Medicine·Monica MuratoriElisabetta Baldi
Feb 26, 2020·International Journal of Molecular Sciences·Marion DelessardNathalie Rives
Jul 15, 2020·Annual Review of Genetics·R John AitkenBrett Nixon
Dec 22, 2009·Reproductive Biomedicine Online·Francesco M LanzafameAldo E Calogero

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.