DNA synthesis and related enzymes altered in compensatory lung growth in rats

Scandinavian Journal of Clinical and Laboratory Investigation
S KuboiK Kida

Abstract

Left pneumonectomy was performed on 4 week-old male Fischer-344 rats. Changes in DNA biosynthesis and the activities of related enzymes were studied in the contralateral lungs of the pneumonectomized animals (n = 55) and compared with sham-operated (n = 55) and untreated control animals (n = 40) The wet weight of the contralateral lung of the pneumonectomized rats reached that of both lungs of the untreated and sham-operated rats 14 days after the operation. The activities of thymidine kinase and DNA polymerase from the regenerating lungs were elevated on Days 1 and 7. To determine the molecular forms of DNA polymerase in the crude extract, phosphocellulose column chromatography was performed. The type of DNA polymerase with the highest activity was alpha in regenerating lung on Days 1, 3, and 7. These results suggest that DNA replication for cellular proliferation was elevated in the remaining lung after pneumonectomy. In addition, an interlobar difference in DNA biosynthesis was observed in the remaining lung. The increase was especially marked in the cardiac lobe, followed by increases in the DNA content of the remaining lobes on Day 7. From these observations we conclude (1) that increased activity of DNA polymerase alpha i...Continue Reading

References

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Citations

Sep 7, 2007·Cell and Tissue Research·Darrell N Kotton, Alan Fine
Dec 18, 2013·Pharmacology & Therapeutics·Derek PaisleyCarina Gross
Apr 29, 1998·Experimental Lung Research·W A PriceA D Stiles
Nov 3, 2007·The Journal of Thoracic and Cardiovascular Surgery·Lucas G FernandezVictor E Laubach
Jan 30, 2007·The Journal of Thoracic and Cardiovascular Surgery·Lucas G FernandezVictor E Laubach
Aug 1, 1995·The Journal of Thoracic and Cardiovascular Surgery·B A DubayboG J Rubeiz
Oct 1, 1994·Scandinavian Journal of Clinical and Laboratory Investigation·T MizuuchiK Kida

Related Concepts

Metazoa
DNA, Double-Stranded
DNA Polymerase I
DNA Polymerase II
Lung
Lung Volume Reduction
Rats, Inbred F344
Thymidine Kinase
Rats, Laboratory

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