DNA synthesis and scanning electron microscopic lesions in renal pelvic epithelium of rats treated with bladder cancer promoters

Toxicology Letters
M A ShibataS Fukushima

Abstract

The proliferation response of rat renal pelvic epithelium, lined by transitional epithelium, to administration of various bladder cancer promoters was investigated. In addition, prostaglandin E2 (PGE2), lipid peroxide (LPO), malondialdehyde (MDA) and cyclic adenosine 3':5'-monophosphate (cyclic AMP) levels were assessed in urine of rats given the non-promoter L-ascorbic acid (AsA) and the promoters sodium L-ascorbate (AsA-Na) or sodium bicarbonate (NaHCO3) for 4 or 8 weeks. DNA synthesis in the renal pelvic epithelium, as assessed by 5-bromo-2'-deoxyuridine (BrdU) incorporation, was increased in the groups given AsA-Na, an extremely high dose of sodium chloride (NaCl), tert-butylhydroquinone (TBHQ) or ethoxyquin (EQ). Moreover, with the exception of AsA-Na, all treatments that induced an elevation of DNA synthesis also caused morphological epithelial alterations as observed by scanning electron microscopy (SEM). Treatment with butylated hydroxyanisole (BHA) and butylated hydroxytoluene (BHT) did not result in any proliferative response of the rat renal pelvis. No treatment-related changes in urinary PGE2 and cyclic AMP were noted, although AsA-Na and AsA but not NaHCO3 reduced levels of LPO and MDA in the urine. The results ind...Continue Reading

References

Jun 1, 1989·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·M A ShibataS Fukushima
May 1, 1987·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·T FujiiK Hiraga
Oct 1, 1986·Carcinogenesis·T H RushmoreM Taningher
Oct 1, 1986·Toxicology and Applied Pharmacology·S L JohanssonS M Cohen
Mar 1, 1982·The American Journal of Anatomy·R Verani, R E Bulger
Aug 1, 1982·Toxicology Letters·G MurasakiS M Cohen
Jan 1, 1980·Toxicology and Applied Pharmacology·D L ArnoldI C Munro

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Citations

Feb 1, 1997·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·A J Cohen, F J Roe
Oct 25, 2002·International Journal of Toxicology·Rebecca S Lanigan, Torill A Yamarik

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