DNA synthesis for true random number generation

Nature Communications
Linda C MeiserRobert N Grass

Abstract

The volume of securely encrypted data transmission required by today's network complexity of people, transactions and interactions increases continuously. To guarantee security of encryption and decryption schemes for exchanging sensitive information, large volumes of true random numbers are required. Here we present a method to exploit the stochastic nature of chemistry by synthesizing DNA strands composed of random nucleotides. We compare three commercial random DNA syntheses giving a measure for robustness and synthesis distribution of nucleotides and show that using DNA for random number generation, we can obtain 7 million GB of randomness from one synthesis run, which can be read out using state-of-the-art sequencing technologies at rates of ca. 300 kB/s. Using the von Neumann algorithm for data compression, we remove bias introduced from human or technological sources and assess randomness using NIST's statistical test suite.

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Citations

Apr 10, 2021·Nucleic Acids Research·Chengtao XuHong Liu
Oct 12, 2021·Chemistry : a European Journal·Yuxin LiuFelix F Loeffler

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Methods Mentioned

BETA
PCR
chip
Illumina sequencing
DNA concentration assay

Software Mentioned

python
Matlab
Mersenne Twister
Microsynth Genomics
HotBits
RNG
Microsynth

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