Abstract
Escherichia coli cells infected with gene H mutants of bacteriophage phi X174 produce two types of particles. The 110S particles contain single-stranded circular DNA; the 110S particles are not infectious, although their DNA is infectious for E. coli spheroplasts. The second type of particles, 70S particles, contain a fragment of single-stranded DNA ranging from 0.2 to 0.5 genome in length. This fragment DNA anneals only to restriction enzyme fragments of replicative-form DNA from the portion of the molecule corresponding to the origin and early region of phi X174 single-stranded synthesis, although full-round single-stranded DNA synthesis is occurring in the H mutant-infected cells. Different H mutant phages produce different proportions of 70S to 110S particles; those mutants producing the most 70S also exhibit the largest amount of degradation of intracellularly labeled DNA during infection. These results suggest that in H mutant-infected cells, full-length single-stranded DNA is synthesized; varying amounts of degradation of the single-stranded material occur, and the resulting fragment DNA is subsequently incorporated into 70S particles.
References
Jun 1, 1977·Journal of Virology·R Mukai, M Hayashi
Feb 1, 1977·Journal of Virology·H Fujisawa, M Hayashi
Feb 24, 1977·Nature·F SangerM Smith
Aug 1, 1977·Journal of Virology·H Fujisawa, M Hayashi
Oct 1, 1977·Journal of Virology·H Fujisawa, M Hayashi
Aug 1, 1976·Journal of Virology·H Fujisawa, M Hayashi
Jul 1, 1975·Virology·S M JazwinskiA Kornberg
Jul 1, 1975·Virology·S M JazwinskiA Kornberg
Dec 1, 1975·CRC Critical Reviews in Microbiology·D T Denhardt
Jun 14, 1967·Journal of Molecular Biology·B Hirt
May 1, 1972·Virology·P J WeisbeekG A Van Arkel
Nov 1, 1974·Journal of Virology·M N Hayashi, M Hayashi
Nov 25, 1974·Journal of Molecular Biology·G N Godson
Sep 28, 1969·Journal of Molecular Biology·D H Gelfand, M Hayashi
Apr 28, 1971·Journal of Molecular Biology·M Iwaya, D T Denhardt
May 1, 1971·Journal of Virology·R BenzingerR Huskey
Jun 10, 1968·Biochemical and Biophysical Research Communications·J E SiegelM Hayashi
Oct 1, 1969·Journal of Virology·J E Siegel, M Hayashi
Citations
Jul 31, 2009·Journal of Virology·Mark V RuboyianesBentley A Fane
Jan 1, 1988·Molecular & General Genetics : MGG·R K HamatakeM Hayashi
Nov 29, 2013·Journal of Virology·Lindsey N YoungBentley A Fane
Jul 1, 2016·Journal of Virology·Aaron P Roznowski, Bentley A Fane
Dec 9, 2003·Journal of Molecular Biology·Christopher R Novak, Bentley A Fane