PMID: 376874Mar 1, 1979Paper

DNA synthesis in Escherichia coli cells infected with gene H mutants of bacteriophage phi X174

Journal of Virology
K R Spindler, M Hayashi

Abstract

Escherichia coli cells infected with gene H mutants of bacteriophage phi X174 produce two types of particles. The 110S particles contain single-stranded circular DNA; the 110S particles are not infectious, although their DNA is infectious for E. coli spheroplasts. The second type of particles, 70S particles, contain a fragment of single-stranded DNA ranging from 0.2 to 0.5 genome in length. This fragment DNA anneals only to restriction enzyme fragments of replicative-form DNA from the portion of the molecule corresponding to the origin and early region of phi X174 single-stranded synthesis, although full-round single-stranded DNA synthesis is occurring in the H mutant-infected cells. Different H mutant phages produce different proportions of 70S to 110S particles; those mutants producing the most 70S also exhibit the largest amount of degradation of intracellularly labeled DNA during infection. These results suggest that in H mutant-infected cells, full-length single-stranded DNA is synthesized; varying amounts of degradation of the single-stranded material occur, and the resulting fragment DNA is subsequently incorporated into 70S particles.

References

Jun 1, 1977·Journal of Virology·R Mukai, M Hayashi
Feb 1, 1977·Journal of Virology·H Fujisawa, M Hayashi
Feb 24, 1977·Nature·F SangerM Smith
Aug 1, 1977·Journal of Virology·H Fujisawa, M Hayashi
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Jun 10, 1968·Biochemical and Biophysical Research Communications·J E SiegelM Hayashi

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Citations

Jan 1, 1988·Molecular & General Genetics : MGG·R K HamatakeM Hayashi
Jul 1, 2016·Journal of Virology·Aaron P Roznowski, Bentley A Fane

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