DNA synthesis increases during the first hours post-emergence in Anopheles albimanus mosquito midgut.

Developmental and Comparative Immunology
Krystal Maya-MaldonadoHumberto Lanz-Mendoza

Abstract

In hematophagous insects, the midgut is a fundamental barrier against infections and limits the development and transmission of pathogens. However, in mosquitoes, cell differentiation, proliferation, and cell cycle process in the midgut have not been characterized. Here we provide evidence of how cell cycle progression occurs in the newly emerged Anopheles albimanus mosquito midgut and describing cyclins expression as mediators of the cell cycle. The cell cycle at different post-emergence times was evaluated in disaggregated cells from midgut tissue using flow cytometry. Also, cyclins A, B, and E were identified by bioinformatics tools. These cyclins were used to analyze cell cycle progression. Flow cytometry data and the expression-pattern of the cyclins by qRT-PCR supported a polyploidy process, besides mitosis marker was marginally detected and only in newly emerged mosquitoes. Our results suggest that DNA increment in midguts occurs by polyploidy during the first hours post-emergence.

Citations

Apr 13, 2021·Frontiers in Cellular and Infection Microbiology·Bretta HixsonNicolas Buchon

❮ Previous
Next ❯

Related Concepts

Related Feeds

Related Papers

Current Biology : CB
C Norbury, P Nurse
Current Topics in Microbiology and Immunology
J Hanley-Hyde
Cytometry
Z DarzynkiewiczF Traganos
© 2022 Meta ULC. All rights reserved