PMID: 3750314Jan 1, 1986Paper

DNA synthesis of adult mammalian cardiac muscle cells in long-term culture

Tissue & Cell
A C Nag, M Cheng

Abstract

Adult rat cardiac ventricular muscle cells were isolated and cultured in monolayer for 30-45 days. Most of the cardiac muscle cells undergo external and internal structural alterations, resembling embryonic/neonatal cardiac muscle cells in culture (Nag and Cheng, 1981; Nag et al., 1983). These cultured cells underwent DNA synthesis and mitosis as revealed by autoradiography studies that involved the exposure of the cells to [3H]-thymidine for 24 hr prior to the termination of the culture at selected intervals. During the first week of culture, cardiac muscle cells showed less than 5% labeled cells. The labeling index of myocytes attained a peak in the second week of culture, exhibiting approximately 23% labeled cells. The labeling indices of cardiac muscle cells declined over the period of 30 days of culture. During the end of the incubation period, approximately 4% of the myocytes were labeled. When the extent of the total cell population involved in DNA synthesis was examined by exposing the cells to [3H]-thymidine continuously for long periods of time, it was observed that approximately 26% of the cardiac muscle cells regained the capacity for DNA synthesis during 1-10 days of culture. From day 1 to day 14, approximately 29%...Continue Reading

References

Feb 1, 1973·Experimental Cell Research·I S Polinger
Feb 1, 1974·The Journal of Experimental Zoology·J O Oberpriller, J C Oberpriller
Jan 1, 1981·Tissue & Cell·A C Nag, M Cheng

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