PMID: 590657Oct 13, 1977

DNA synthetic capabilities of differentiating sperm cells

Differentiation; Research in Biological Diversity
D L DaentlC J Betlach


Spermatogenic cells separated by velocity sedimentation were analysed by a micro-procedure for differentiation-associated changes in DNA synthetic capabilities. DNA-dependent DNA polymerase activity is maximal in premeiotic and meiotic cells, sequentially declines in progressively more differentiated spermiogenic cells to a minimum value in testicular spermatozoa which is 1/14 of the maximum. No further decrease of activity is observed during the subsequent process of sperm cell maturation and, at the end-differentiated state, the potential of sperm cells for DNA synthesis is demonstrated by the presence of substantial activities of thymidine and thymidylate kinases as well as DNA polymerase activity, as determined by in vitro assay, are polymerase. Although levels of DNA polymerase activity, as determined by in vitro assay, are negatively correlated with the state of differentiation, the findings support the hypothesis that, in this cell system, DNA synthetic enzymes may not be limiting factors in the control of DNA synthesis.


Jan 1, 1975·The Journal of Cell Biology·M L MeistrichW J Barcellona
Jan 1, 1970·Archives of Biochemistry and Biophysics·J G GeorgatsosC Gabrielides
Dec 1, 1974·Proceedings of the National Academy of Sciences of the United States of America·G A Sega
Dec 1, 1974·Journal of Reproduction and Fertility·N B Hecht
Feb 1, 1972·Proceedings of the National Academy of Sciences of the United States of America·B FridlenderA Weissbach
Oct 11, 1972·Nature: New Biology·J F Chiu, S C Sung
Mar 1, 1973·The Journal of Experimental Zoology·B L Gledhill, Z Darzynkiewicz
Feb 15, 1973·Journal of Molecular Biology·L M ChangF J Bollum
Jun 1, 1974·Experimental Cell Research·R P Erickson
Mar 1, 1972·The Journal of Cell Biology·F E Stockdale, M C O'Neill
May 29, 1972·Biochimica Et Biophysica Acta·J F Chiu, S C Sung
Jul 25, 1972·Biochemical and Biophysical Research Communications·P Grippo, A L Scavo
Nov 1, 1971·Experimental Cell Research·S Kofman-Alfaro, A C Chandley
Nov 1, 1971·Experimental Cell Research·A C Chandley, S Kofman-Alfaro
Sep 1, 1968·Proceedings of the National Academy of Sciences of the United States of America·R R Meyer, M V Simpson
Jan 1, 1970·Proceedings of the National Academy of Sciences of the United States of America·D M LamW R Bruce
Jan 1, 1970·Chromosoma·S Kofman-Alfaro, A C Chandley
Jul 22, 1969·Biochimica Et Biophysica Acta·H R Woodland
Jan 1, 1963·Experimental Cell Research· VAN POTTER
Oct 1, 1964·Journal of Chromatography·K RANDERATH, E RANDERATH


Oct 1, 1978·Cell Differentiation·P GrippoV MONESI
Sep 1, 1981·Journal of Reproductive Immunology·R P EricksonM Butley
Jul 1, 1989·Cell Differentiation and Development : the Official Journal of the International Society of Developmental Biologists·P OrlandoP Grippo
Jul 29, 1980·Biochimica Et Biophysica Acta·A HabaraY Mano
Jan 1, 1989·Archives of Andrology·M FujisawaS Kamidono

Related Concepts

Cell Nucleus
Cytoplasmic Matrix
DNA, Double-Stranded
Y-Chromosome-Bearing Sperm
Thymidine Kinase

Trending Feeds


Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Genetic Screens in iPSC-derived Brain Cells

Genetic screening is a critical tool that can be employed to define and understand gene function and interaction. This feed focuses on genetic screens conducted using induced pluripotent stem cell (iPSC)-derived brain cells. It also follows CRISPR-Cas9 approaches to generating genetic mutants as a means of understanding the effect of genetics on phenotype.

Alzheimer's Disease: MS4A

Variants within the membrane-spanning 4-domains subfamily A (MS4A) gene cluster have recently been implicated in Alzheimer's disease in genome-wide association studies. Here is the latest research on Alzheimer's disease and MS4A.

Pediculosis pubis

Pediculosis pubis is a disease caused by a parasitic insect known as Pthirus pubis, which infests human pubic hair, as well as other areas with hair including eye lashes. Here is the latest research.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Rh Isoimmunization

Rh isoimmunization is a potentially preventable condition that occasionally is associated with significant perinatal morbidity or mortality. Discover the latest research on Rh Isoimmunization here.

Pharmacology of Proteinopathies

This feed focuses on the pharmacology of proteinopathies - diseases in which proteins abnormally aggregate (i.e. Alzheimer’s, Parkinson’s, etc.). Discover the latest research in this field with this feed.

Enzyme Evolution

This feed focuses on molecular models of enzyme evolution and new approaches (such as adaptive laboratory evolution) to metabolic engineering of microorganisms. Here is the latest research.

Alignment-free Sequence Analysis Tools

Alignment-free sequence analyses have been applied to problems ranging from whole-genome phylogeny to the classification of protein families, identification of horizontally transferred genes, and detection of recombined sequences. Here is the latest research.