PMID: 9434158Jan 20, 1998Paper

DNA topoisomerase II cleavage of telomeres in vitro and in vivo

Biochimica Et Biophysica Acta
H J YoonI K Chung

Abstract

In this work, we have analyzed the reactivity of DNA topoisomerase II with telomeric DNA both in vitro and in vivo. Topoisomerase II cleavage reactions were performed on the tandem repeats of telomeric DNA. Analysis of this DNA on sequencing gels revealed that DNA topoisomerase II is catalytically active in cleaving the telomere DNA repeat. The topoisomerase II cleavage site is 5'TTAGG*G3' (cleavage site marked by the asterisk) and since telomere DNA is a tandem array of the above sequence, topoisomerase cleavage sites could exist every six base pairs. Detection of topoisomerase II cleavages was strongly dependent upon one specific topoisomerase II poison, etoposide (VP-16). A number of other topoisomerase II poisons were tested but did not stimulate cleavage activity at the telomere repeat. We have also analyzed the association of endogenous topoisomerase II with chromosomal telomeric DNA in HeLa cells. The in vivo complex of enzyme (ICE) bioassay was used to isolate topoisomerase II-DNA covalent complexes. In consistence with in vitro cleavage data, endogenous topoisomerase II-telomeric DNA complexes were detected in only etoposide-treated HeLa cells.

References

Apr 25, 1992·Nucleic Acids Research·I K ChungM T Muller
Nov 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·R C AllsoppC B Harley
Apr 18, 1991·Nature·E H Blackburn
Jan 11, 1990·Nucleic Acids Research·J R SpitznerM T Muller
May 31, 1990·Nature·C B HarleyC W Greider
Dec 17, 1989·Biochimica Et Biophysica Acta·P D'Arpa, L F Liu
Apr 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·Y S BaeL F Liu
Jun 24, 1988·Nucleic Acids Research·J R Spitzner, M T Muller
Nov 1, 1988·Biochemistry·M T MullerK Tsutsui
May 1, 1985·The Journal of Cell Biology·W C EarnshawL F Liu
May 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·S DiNardoR Sternglanz
Apr 1, 1995·Current Opinion in Genetics & Development·C B Harley, B Villeponteau
Sep 1, 1994·Molecular and Cellular Biology·H TommerupT de Lange
Sep 1, 1994·Molecular and Cellular Biology·J W Phillips, W F Morgan
Apr 12, 1994·Proceedings of the National Academy of Sciences of the United States of America·C M CounterC B Harley
Aug 23, 1996·The Journal of Biological Chemistry·J S KingW F Morgan
Jan 1, 1996·Annual Review of Biochemistry·C W Greider
Jan 1, 1996·Annual Review of Biochemistry·J C Wang

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Citations

May 22, 2003·Cancer Letters·Gabriele Saretzki
Jan 23, 2002·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·C Hennequin, V Favaudon
May 9, 2000·Critical Reviews in Oncology/hematology·F LavelleP Mailliet
Jan 11, 2000·Nucleic Acids Research·P B ArimondoC Hélène
Jan 11, 2014·Mutation Research. Fundamental and Molecular Mechanisms of Mutagenesis·Natalia S PavioloAlejandro D Bolzán
Feb 14, 2002·Nucleic Acids Research·Jean-Louis MergnyEric Gilson
Jun 26, 2012·The International Journal of Biochemistry & Cell Biology·Ping LiDawei Xu
Mar 7, 2003·Molecular Cell·Kyle M Miller, Julia Promisel Cooper
May 30, 2012·The Journal of Cell Biology·Joseph K HsuRobert Y L Tsai
Dec 29, 2005·International Journal of Cancer. Journal International Du Cancer·Jessie C JeyapalanThomas von Zglinicki
Jul 12, 2002·Molecular Carcinogenesis·Rosemary FinnonAndrew Silver
Jan 20, 2004·The Journal of Biological Chemistry·Mi Ran KangIn Kwon Chung
Dec 12, 2002·The Journal of Biological Chemistry·Violeta SerraGabriele Saretzki
Jul 27, 2000·Annals of the New York Academy of Sciences·T von Zglinicki
Feb 14, 2006·Critical Reviews in Oncology/hematology·Ken André OlaussenJean-Charles Soria

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