DNA translocation governed by interactions with solid-state nanopores.

Biophysical Journal
Meni WanunuAmit Meller

Abstract

We investigate the voltage-driven translocation dynamics of individual DNA molecules through solid-state nanopores in the diameter range 2.7-5 nm. Our studies reveal an order of magnitude increase in the translocation times when the pore diameter is decreased from 5 to 2.7 nm, and steep temperature dependence, nearly threefold larger than would be expected if the dynamics were governed by viscous drag. As previously predicted for an interaction-dominated translocation process, we observe exponential voltage dependence on translocation times. Mean translocation times scale with DNA length by two power laws: for short DNA molecules, in the range 150-3500 bp, we find an exponent of 1.40, whereas for longer molecules, an exponent of 2.28 dominates. Surprisingly, we find a transition in the fraction of ion current blocked by DNA, from a length-independent regime for short DNA molecules to a regime where the longer the DNA, the more current is blocked. Temperature dependence studies reveal that for increasing DNA lengths, additional interactions are responsible for the slower DNA dynamics. Our results can be rationalized by considering DNA/pore interactions as the predominant factor determining DNA translocation dynamics in small por...Continue Reading

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Citations

Jun 10, 2009·European Biophysics Journal : EBJ·Kozhinjampara R MahendranMathias Winterhalter
Dec 16, 2010·Journal of the American Chemical Society·Meni WanunuMarija Drndic
Jan 11, 2012·Nano Letters·Stefan W KowalczykCees Dekker
Mar 1, 2012·Nano Letters·C RaillonA Radenovic
Feb 23, 2012·Nano Letters·Alon SingerAmit Meller
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Nov 10, 2011·ACS Nano·Meni WanunuMarija Drndic
Oct 3, 2013·ACS Nano·Joseph LarkinMeni Wanunu
Sep 16, 2009·ACS Nano·Vincent Tabard-CossaAndre Marziali
Mar 20, 2012·Nature Methods·Jacob K RosensteinKenneth L Shepard
Dec 22, 2009·Nature Nanotechnology·Meni WanunuAmit Meller
Oct 26, 2010·Nature Nanotechnology·Meni WanunuMarija Drndić
Nov 30, 2010·Nature Nanotechnology·Adam R HallCees Dekker
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