PMID: 2492487Jan 1, 1989Paper

DNA typing of DRw6 subtypes: correlation with DRB1 and DRB3 allelic sequences by hybridization with oligonucleotide probes

Human Immunology
J M TiercyB Mach

Abstract

Among MHC class II antigens, the DRw6/Dw6 complex represents a special situation where typing on a routine basis is often troublesome, mainly because monospecific alloantisera are rare and individual subtypes numerous. We demonstrate here that the use of oligonucleotide DNA typing permits an analysis of the polymorphism within DRw6 haplotypes and provides a molecular basis for correlations with functional data. Synthetic oligonucleotide probes, most of them locus- and allele-specific, were derived from the DNA sequences of three alleles of locus DRB1 and three alleles of locus DRB3. These probes allow the positive identification of distinct DRw6 subtypes. As analyzed on a panel of 26 well-defined DRw6 cell lines, oligotyping allows a direct and absolute correlation with the DRw13 serologic specificity and with the cellularly defined Dw9,Dw16,Dw18, and Dw19 specificities. Correlations of the polymorphism at the DRB1 locus with the polymorphism at the DRB3 locus (DRw52 alleles) allow us to identify preferential allelic associations such as DRw13-Dw18-DRw52a/52b, DRw13-Dw19-DRw52c, and DRw13/Dw19 haplotype, the Dw19 cellular reactivity might involve, at least DRw14-Dw9-DRw52b. In view of the absolute segregation of the DRw52c alle...Continue Reading

References

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Citations

Jan 1, 1990·Immunogenetics·R SorrentinoN Tanigaki
Jan 1, 1990·Rheumatology International·B LangH H Peter
Jan 1, 1990·Immunologic Research·R Lechler, G Lombardi
Jul 1, 1990·Human Immunology·J G BodmerT Sasazuki
May 1, 1990·Human Immunology·A Termijtelen
Apr 1, 1991·Human Immunology·B Mach, J M Tiercy
May 14, 1996·Proceedings of the National Academy of Sciences of the United States of America·D G ForcioneS Pillai
Mar 5, 2003·The Journal of Experimental Medicine·Linh LuJohn Kappler
Aug 1, 1997·European Journal of Immunogenetics : Official Journal of the British Society for Histocompatibility and Immunogenetics·N Martinez-QuilesA Arnaiz-Villena
Jan 1, 1990·Tissue Antigens·J G BodmerT Sasazuki
Feb 1, 1991·Tissue Antigens·E ReedN Suciu-Foca
Feb 1, 1991·European Journal of Immunogenetics : Official Journal of the British Society for Histocompatibility and Immunogenetics·R W Vaughan
Jun 1, 1991·European Journal of Immunogenetics : Official Journal of the British Society for Histocompatibility and Immunogenetics·J D Bignon, J L Bidwell
Oct 1, 1991·European Journal of Immunogenetics : Official Journal of the British Society for Histocompatibility and Immunogenetics·C Nevinny-StickelE D Albert
Oct 1, 1992·European Journal of Immunogenetics : Official Journal of the British Society for Histocompatibility and Immunogenetics·A AmarA G Smith
Dec 1, 1992·European Journal of Immunogenetics : Official Journal of the British Society for Histocompatibility and Immunogenetics·U R SorgP Wernet
Jun 1, 1994·European Journal of Immunogenetics : Official Journal of the British Society for Histocompatibility and Immunogenetics·C GrundschoberJ M Tiercy
Dec 1, 1989·Clinical Immunology and Immunopathology·R Wassmuth, A Lernmark

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