PMID: 9174135May 1, 1997Paper

DNA typing of human platelet antigen systems 1, 2, 3 and 5 in B-lymphoblastoid cell lines of the International Histocompatibility Workshop

Tissue Antigens
G BeinH Klüter

Abstract

Alloimmunization against human platelet antigens (HPA) can cause thrombocytopenia in different clinical settings, including neonatal alloimmune thrombocytopenia, post transfusion purpura, and refractoriness to platelet transfusion. Recently, DNA based methods have been described for analysis of the polymorphism of the human platelet antigens. However, until now, no reference material for standardization purposes is available. We thus determined the DNA polymorphism of the human platelet antigen (HPA) systems 1, 2, 3 and 5 in B-lymphoblastoid cell lines collected for the International Histocompatibility Workshops. DNA typing of the HPA systems was done by PCR amplification with sequence-specific primers (PCR-SSP). A new enzyme (AmpliTaq Gold) was introduced to enhance the specificity of the PCR. We present a panel of five B-lymphoblastoid cell lines, representing all heterozygous and homozygous genotypes of the tested HPA systems. This work thus provides the reference material required for DNA based diagnosis of human platelet antigens.

Citations

Nov 18, 1998·Journal of Immunological Methods·C FrohnH Kirchner
Mar 24, 2000·Transfusion clinique et biologique : journal de la Société française de transfusion sanguine·A DormoyJ P Cazenave
Aug 15, 1998·Vox Sanguinis·H KrollS Santoso
Dec 17, 2003·European Journal of Immunogenetics : Official Journal of the British Society for Histocompatibility and Immunogenetics·D C JonesS E Marshall
Sep 26, 2009·Transfusion Medicine·M PavicS Dodig
Sep 8, 2004·Transplantation Proceedings·E M KotzampasakiC Stavropoulos-Giokas
Dec 3, 1999·European Journal of Immunogenetics : Official Journal of the British Society for Histocompatibility and Immunogenetics·J SellersC Darke
Dec 9, 2015·Scientific Reports·Femke E van BeekAstrid M L Kappers
May 16, 1998·Tissue Antigens·Y W ChangB R Hawkins

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