DNA vaccination by immersion and ultrasound to trout viral haemorrhagic septicaemia virus

Vaccine
M Fernandez-AlonsoJ M Coll

Abstract

This work reports preliminary data on the application of a novel method, ultrasound, for the DNA vaccination of rainbow trout. First, the best formulations were selected that increased the transfer by immersion of a plasmid coding for the green fluorescent protein (GFP) gene into trout fry. Quantification of GFP expression by fluorescence in the fin cells was used to study time course, DNA concentration dependence and comparison of different formulations. The best GFP expression results were obtained with short pulses of ultrasound, DOTAP liposomes and recombinant bacteria or bactofection. Other liposomes or microencapsulation formulations resulted in a GFP fluorescence similar to background values. Second, DNA immersion-vaccination of immunocompetent fingerling trout with the selected formulations was performed by using a plasmid coding for the glycoprotein G gene of the viral haemorrhagic septicaemia virus (VHSV). The immunization of fingerling trout was estimated by measuring humoral antibody, lymphoproliferation and VHSV challenge responses. Short pulses of low intensity ultrasound were the only method by which both humoral antibody responses and survival after VHSV challenge were obtained. Immersion DNA-vaccination using s...Continue Reading

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Citations

Mar 10, 2005·Marine Biotechnology·Erwin A RamosCelia Aurora T Torres-Villanueva
Apr 12, 2002·Veterinary Immunology and Immunopathology·A RochaJ M Coll
Mar 19, 2011·Developmental and Comparative Immunology·Karen P Plant, Scott E Lapatra
May 2, 2008·Fish & Shellfish Immunology·Tom Christian TonheimRoy Ambli Dalmo
Jan 31, 2006·Journal of Biotechnology·C TafallaJ M Coll
Jul 26, 2012·Expert Review of Vaccines·Karen Smith KorsholmDennis Christensen
Nov 18, 2008·International Journal of Pharmaceutics·Afendi DahlanSudaxshina Murdan
Apr 30, 2003·Expert Opinion on Biological Therapy·Nicole Huebener, Holger N Lode
Apr 30, 2003·Expert Opinion on Biological Therapy·Wolfgang W Leitner
Feb 22, 2012·Veterinary Immunology and Immunopathology·Jason P Evenhuis, Beth M Cleveland
Jun 19, 2017·Fish & Shellfish Immunology·Julio Coll
Apr 15, 2003·The Journal of General Virology·Douglas J LeisyGeorge F Rohrmann
Apr 10, 2021·PloS One·Carmen Navarro-GuillénSofia Engrola

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