DNA vaccine-generated duck polyclonal antibodies as a postexposure prophylactic to prevent hantavirus pulmonary syndrome (HPS).

PloS One
R L BrocatoJ W Hooper

Abstract

Andes virus (ANDV) is the predominant cause of hantavirus pulmonary syndrome (HPS) in South America and the only hantavirus known to be transmitted person-to-person. There are no vaccines, prophylactics, or therapeutics to prevent or treat this highly pathogenic disease (case-fatality 35-40%). Infection of Syrian hamsters with ANDV results in a disease that closely mimics human HPS in incubation time, symptoms of respiratory distress, and disease pathology. Here, we evaluated the feasibility of two postexposure prophylaxis strategies in the ANDV/hamster lethal disease model. First, we evaluated a natural product, human polyclonal antibody, obtained as fresh frozen plasma (FFP) from a HPS survivor. Second, we used DNA vaccine technology to manufacture a polyclonal immunoglobulin-based product that could be purified from the eggs of vaccinated ducks (Anas platyrhynchos). The natural "despeciation" of the duck IgY (i.e., Fc removed) results in an immunoglobulin predicted to be minimally reactogenic in humans. Administration of ≥ 5,000 neutralizing antibody units (NAU)/kg of FFP-protected hamsters from lethal disease when given up to 8 days after intranasal ANDV challenge. IgY/IgYΔFc antibodies purified from the eggs of DNA-vaccina...Continue Reading

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Citations

Oct 9, 2012·Nature Medicine·Elie Dolgin
Jun 19, 2012·Antiviral Research·David SafronetzJay W Hooper
Aug 13, 2015·BioMed Research International·Joseph W GoldenRebecca L Brocato
Jan 23, 2014·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·J W HooperC Schmaljohn
Jul 26, 2018·Journal of Immunoassay & Immunochemistry·Natália Maria LanzariniMarcelo Alves Pinto
Dec 15, 2012·Clinical and Vaccine Immunology : CVI·R L BrocatoJ W Hooper
Jul 17, 2013·Antimicrobial Agents and Chemotherapy·David SafronetzBrian B Gowen
Nov 23, 2018·Science Translational Medicine·Jose L GarridoMaria I Barria
Sep 8, 2020·Clinical and Experimental Pharmacology & Physiology·Naveed MunirAneezah Sana
Feb 23, 2020·Frontiers in Microbiology·Rongrong LiuXingan Wu
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Sep 3, 2020·Vaccines·José M Pérez de la LastraAntonio Morales-delaNuez
Jul 7, 2019·Viruses·Rebecca L Brocato, Jay W Hooper
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Methods Mentioned

BETA
ELISA
ELISAs

Software Mentioned

GraphPad Prism
Synagis
GeneSnap

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