DNA Vaccines Encoding Toxoplasma gondii Cathepsin C 1 Induce Protection against Toxoplasmosis in Mice

The Korean Journal of Parasitology
Yali HanShenyi He

Abstract

Toxoplasma gondii cathepsin C proteases (TgCPC1, 2, and 3) are important for the growth and survival of T. gondii. In the present study, B-cell and T-cell epitopes of TgCPC1 were predicted using DNAstar and the Immune Epitope Database. A TgCPC1 DNA vaccine was constructed, and its ability to induce protective immune responses against toxoplasmosis in BALB/c mice was evaluated in the presence or absence of the adjuvant α-GalCer. As results, TgCPC1 DNA vaccine with or without adjuvant α-GalCer showed higher levels of IgG and IgG2a in the serum, as well as IL-2 and IFN-γ in the spleen compared to controls (PBS, pEGFP-C1, and α-Galcer). Upon challenge infection with tachyzoites of T. gondii (RH), pCPC1/α-Galcer immunized mice showed the longest survival among all the groups. Mice vaccinated with DNA vaccine without adjuvant (pCPC1) showed better protective immunity compared to other controls (PBS, pEGFP-C1, and α-Galcer). These results indicate that a DNA vaccine encoding TgCPC1 is a potential vaccine candidate against toxoplasmosis.

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Citations

Aug 24, 2018·PLoS Neglected Tropical Diseases·Jair L Siqueira-NetoPhilip J Rosenthal
Jul 13, 2018·The Korean Journal of Parasitology·Zhong-Yuan LiXing-Quan Zhu
Dec 7, 2018·Parasitology Research·Carlos J Ramírez-FloresRicardo Mondragón-Flores

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Methods Mentioned

BETA
PCR
transfection
fluorescence microscopy
ELISA

Software Mentioned

DNAStar
SPSS

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