DNA vaccines expressing antigens with a stress protein-capturing domain display enhanced immunogenicity

Immunological Reviews
Jörg Reimann, Reinhold Schirmbeck

Abstract

An expression system for DNA vaccines is described, in which a fusion protein with an N-terminal, viral J-domain that captures heat-shock proteins (Hsps) is translated in-frame with C-terminal antigen-encoding sequences (of various lengths and origins). The system supports enhanced expression of chimeric antigens (of >800 residues in length) with an extended half life (>8 h). When used as a DNA vaccine, it delivers antigen together with the intrinsic adjuvant activity provided by bound Hsps. We describe the design of vectors for DNA vaccination that support the expression of different immunogenic domains of different origins as large, Hsp-capturing chimeric fusion antigens. The immunogenicity of the antigens produced by this expression system (when it is built into DNA vaccines) has been characterized in detail, with particular emphasis on priming CD8+ T-cell responses. We also discuss areas of vaccine research to which the new technology may provide useful contributions.

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Citations

Jun 14, 2008·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·Andreas WielandReinhold Schirmbeck
Nov 6, 2007·International Journal of Pharmaceutics·Makiya NishikawaYoshinobu Takakura
Dec 29, 2007·The Journal of Pathology·X Zhang, D M Mosser
Feb 18, 2015·PloS One·Margaret Mariscal MonetteLiliana Jaso-Friedmann
Oct 11, 2008·Expert Review of Vaccines·Azam Bolhassani, Sima Rafati
Nov 6, 2009·The Journal of Immunology : Official Journal of the American Association of Immunologists·Helen BrosiReinhold Schirmbeck
Jul 20, 2006·The Journal of Immunology : Official Journal of the American Association of Immunologists·Reinhold SchirmbeckJörg Reimann

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