Abstract
Pneumococcal surface protein A (PspA) is a promising candidate for the development of cost-effective vaccines against Streptococcus pneumoniae. In the present study, BALB/c mice were immunized with DNA vaccine vectors expressing the N-terminal region of PspA. Animals immunized with a vector expressing secreted PspA developed higher levels of antibody than mice immunized with the vector expressing the antigen in the cytosol. However, both immunogens elicited similar levels of protection against intraperitoneal challenge. Furthermore, immunization with exactly the same fragment in the form of a recombinant protein, with aluminium hydroxide as an adjuvant, elicited even higher antibody levels, but this increased humoral response did not correlate with enhanced protection. These results show that DNA vaccines expressing PspA are able to elicit protection levels comparable to recombinant protein, even though total anti-PspA IgG response is considerably lower.
References
Apr 1, 1997·Gene Therapy·L S McDanielD T Curiel
Dec 31, 1997·The Journal of Experimental Medicine·R S ChuC V Harding
Aug 24, 1999·Infection and Immunity·A H TuA J Szalai
Feb 26, 2000·Vaccine·D E BrilesA Swift
Nov 9, 2000·The Journal of Infectious Diseases·D E BrilesG S Nabors
Aug 14, 2001·Infection and Immunity·J R BosargeL S McDaniel
Oct 13, 2001·Infection and Immunity·B P ArulanandamD W Metzger
Aug 17, 2002·Infection and Immunity·Eliane N MiyajiLuciana C C Leite
Mar 18, 2003·Vaccine·Stephen I PeltonHeinz J Schmitt
Apr 16, 2003·The Journal of Infectious Diseases·Maria-Cristina C BrandileoneRobert Austrian
May 29, 2003·FEMS Immunology and Medical Microbiology·Eliane N MiyajiLuciana C C Leite
Sep 27, 2003·Methods : a Companion to Methods in Enzymology·Nicole A Doria-Rose, Nancy L Haigwood
Dec 23, 2003·Infection and Immunity·Bing RenDavid E Briles
May 20, 2004·Vaccine·D BogaertR de Groot
Jul 27, 2004·Brazilian Journal of Medical and Biological Research = Revista Brasileira De Pesquisas Médicas E Biológicas·C R R RamosP L Ho
Aug 24, 2004·Infection and Immunity·Mirza ShaperDavid E Briles
Sep 11, 2004·Biochemical and Biophysical Research Communications·Ana Paula Mattos ArêasPaulo Lee Ho
Citations
Oct 8, 2008·Current Microbiology·Lina LiuJiaqi Tang
Nov 5, 2010·Clinical and Vaccine Immunology : CVI·Jianghong XuXiaoyong Fan
Jun 5, 2010·PloS One·Maria Leonor S OliveiraPaulo L Ho
Apr 13, 2012·PLoS Pathogens·Adam K A WrightStephen B Gordon
Mar 9, 2010·Microbial Pathogenesis·Daniela M FerreiraEliane N Miyaji
Nov 21, 2014·Clinical and Vaccine Immunology : CVI·Naeem KhanDevinder Sehgal
Sep 18, 2012·Microbial Pathogenesis·Cintia F M VadesilhoEliane N Miyaji
Apr 12, 2008·Microbes and Infection·Ivana B CamposMaria Leonor S Oliveira
Jun 29, 2011·International Immunopharmacology·Julio VillenaSusana Alvarez
Mar 10, 2015·Applied Microbiology and Biotechnology·Agnieszka WyszyńskaElżbieta Katarzyna Jagusztyn-Krynicka
Jan 11, 2008·Clinical and Vaccine Immunology : CVI·Daniela M FerreiraEliane N Miyaji
May 18, 2017·Frontiers in Microbiology·Naeem Khan, Arif T Jan
Jun 22, 2010·Molecular Biology Reports·Qiao ZhangChangzheng Wang