PMID: 9539721May 16, 1998Paper

DnaA-stimulated transcriptional activation of orilambda: Escherichia coli RNA polymerase beta subunit as a transcriptional activator contact site

Proceedings of the National Academy of Sciences of the United States of America
A Szalewska-PałaszG Wegrzyn

Abstract

We present evidence that Escherichia coli RNA polymerase beta subunit may be a transcriptional activator contact site. Stimulation of the activity of the pR promoter by DnaA protein is necessary for replication of plasmids derived from bacteriophage lambda. We found that DnaA activates the pR promoter in vitro. Particular mutations in the rpoB gene were able to suppress negative effects that certain dnaA mutations had on the replication of lambda plasmids; this suppression was allele-specific. When a potential DnaA-binding sequence located several base pairs downstream of the pR promoter was scrambled by in vitro mutagenesis, the pR promoter was no longer activated by DnaA both in vivo and in vitro. Therefore, we conclude that DnaA may contact the beta subunit of RNA polymerase during activation of the pR promoter. A new classification of prokaryotic transcriptional activators is proposed.

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Citations

Aug 2, 2007·Journal of Applied Genetics·Agnieszka Szalewska-PalaszAlicja Wegrzyn
May 12, 2011·Archives of Microbiology·Shamlan M S Reshamwala, Santosh B Noronha
May 14, 2003·Journal of Molecular Biology·Monika SłomińskaAlicja Wegrzyn
Dec 15, 2000·Molecular Microbiology·V SperandioJ B Kaper
Jan 4, 2003·Journal of Bacteriology·Saskia VersteegWolfgang Schumann
Nov 18, 2004·Journal of Bacteriology·Anja H SchiemannPaul W O'Toole
Jul 29, 2006·Proceedings of the National Academy of Sciences of the United States of America·Tatiana Venkova-CanovaDhruba K Chattoraj
May 2, 2007·Proceedings of the National Academy of Sciences of the United States of America·Poorna ViswanathanLee Kroos
Feb 7, 2014·Nucleic Acids Research·Paweł OlszewskiMonika Glinkowska
Feb 18, 2005·Journal of Biochemistry and Molecular Biology·Seok Hee Park
Jun 22, 2002·Microbial Cell Factories·Grzegorz Wegrzyn, Alicja Wegrzyn
Mar 23, 2007·Molecular Genetics and Genomics : MGG·Magdalena NarajczykGrzegorz Wegrzyn
Jan 28, 2009·Molecular Microbiology·Ingvild FlåttenKirsten Skarstad
Apr 28, 2007·Microbiology·Magdalena NarajczykGrzegorz Węgrzyn
Mar 26, 2003·The Journal of Biological Chemistry·Monika GlinkowskaGrzegorz Wegrzyn
Oct 24, 1998·FEMS Microbiology Letters·A Szalewska-PałaszG Wegrzyn
Jul 2, 1998·Biochemical and Biophysical Research Communications·A Herman-Antosiewicz, G Wegrzyn
Jun 19, 2021·Frontiers in Microbiology·Inoka P MenikpuragePaola E Mera

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