DNABII proteins play a central role in UPEC biofilm structure

Molecular Microbiology
Aishwarya DevarajSteven D Goodman

Abstract

Most chronic and recurrent bacterial infections involve a biofilm component, the foundation of which is the extracellular polymeric substance (EPS). Extracellular DNA (eDNA) is a conserved and key component of the EPS of pathogenic biofilms. The DNABII protein family includes integration host factor (IHF) and histone-like protein (HU); both are present in the extracellular milieu. We have shown previously that the DNABII proteins are often found in association with eDNA and are critical for the structural integrity of bacterial communities that utilize eDNA as a matrix component. Here, we demonstrate that uropathogenic Escherichia coli (UPEC) strain UTI89 incorporates eDNA within its biofilm matrix and that the DNABII proteins are not only important for biofilm growth, but are limiting; exogenous addition of these proteins promotes biofilm formation that is dependent on eDNA. In addition, we show that both subunits of IHF, yet only one subunit of HU (HupB), are critical for UPEC biofilm development. We discuss the roles of these proteins in context of the UPEC EPS.

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Citations

Aug 12, 2016·Nature Reviews. Microbiology·Hans-Curt FlemmingStaffan Kjelleberg
Feb 14, 2018·Journal of Bacteriology·Christopher J RoccoSteven D Goodman
Sep 26, 2017·Nature Reviews. Microbiology·Hyun KooLuanne Hall-Stoodley
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Nov 27, 2019·Proceedings of the National Academy of Sciences of the United States of America·Aishwarya DevarajSteven D Goodman
Feb 14, 2020·NPJ Biofilms and Microbiomes·Miriam KalsyKrishnendu Mukherjee
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