DnaJ potentiates the interaction between DnaK and alpha-helical peptides

Biochemical and Biophysical Research Communications
A de Crouy-ChanelG Richarme

Abstract

Molecular chaperones bind selectively to nascent, unfolded, misfolded, or aggregated polypeptides, and are involved in protein folding, protein targeting to membranes, and protein renaturation after stress. The DnaK chaperone of Escherichia coli is known to interact preferentially with positively charged hydrophobic peptides in an extended conformation. Accordingly, we show in the present study that DnaK has a low affinity for alpha-helical peptides. In the presence of its co-chaperone DnaJ and ATP, however, DnaK interacts more efficiently with alpha-helical peptides. This suggests that DnaJ triggers a conformational change in DnaK which improves its interaction with these peptides. The ability of the DnaK/DnaJ/GrpE chaperone machine to interact with alpha-helical peptides (which represent the most frequent secondary structure in proteins) should be an important part of its role in protein folding and renaturation.

References

Aug 1, 1992·Trends in Biochemical Sciences·C Georgopoulos
Oct 1, 1992·Journal of Bacteriology·W ShiC A Gross
Apr 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·K LiberekC Georgopoulos
Jul 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·D R PallerosA L Fink
Oct 24, 1991·Nature·G C FlynnJ E Rothman
Jul 1, 1991·Journal of Bacteriology·J Meury, M Kohiyama
Aug 1, 1989·Trends in Biochemical Sciences·R J Ellis, S M Hemmingsen
Jul 3, 1995·Proceedings of the National Academy of Sciences of the United States of America·K LiberekC Georgopoulos
Oct 25, 1994·Proceedings of the National Academy of Sciences of the United States of America·H NagaiT Yura
Jan 1, 1993·Annual Review of Biochemistry·J P Hendrick, F U Hartl
Jan 21, 1994·Journal of Molecular Biology·A GragerovM E Gottesman
Jun 28, 1996·The Journal of Biological Chemistry·A de Crouy-ChanelG Richarme
Sep 1, 1995·Journal of Peptide Science : an Official Publication of the European Peptide Society·O D MoneraR S Hodges

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