DnaJC18, a Novel Type III DnaJ Family Protein, is Expressed Specifically in Rat Male Germ Cells

Balsaeng'gwa saengsig
Cynthia Gomes, Jaemog Soh

Abstract

Mammalian spermatogenesis occurs in a precise and coordinated manner in the seminiferous tubules. One of the attempts to understand the detailed biological process during mammalian spermatogenesis at the molecular level has been to identify the testis specific genes followed by study of the testicular expression pattern of the genes. From the subtracted cDNA library of rat testis prepared using representational difference analysis (RDA) method, a complimentary DNA clone encoding type III member of a DnaJ family protein, DnaJC18, was cloned (GenBank Accession No. DQ158861). The full-length DnaJC18 cDNA has the longest open reading frame of 357 amino acids. Tissue and developmental Northern blot analysis revealed that the DnaJC18 gene was expressed specifically in testis and began to express from postnatal week 4 testis, respectively. In situ hybridization studies showed that DnaJC18 mRNA was expressed only during the maturation stages of late pachy- tene, round and elongated spermatids of adult rat testis. Western blot analysis with DnaJC18 antibody revealed that 41.2 kDa DnaJC18 protein was detected only in adult testis. Immunohistochemistry study further confirmed that DnaJC18 protein, was expressed in developing germ cells an...Continue Reading

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Nov 27, 2004·Biology of Reproduction·Cynthia GomesJaemog Soh
Jan 22, 2005·The EMBO Journal·Kazutoyo TeradaMasataka Mori
Sep 17, 2013·Molecular Reproduction and Development·Sung-Dug OhJaemog Soh
Nov 6, 2015·Physiological Reviews·Michael D Griswold
Mar 24, 2016·Balsaeng'gwa saengsig·Jung Hyun KimHee Jeong Kong

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Methods Mentioned

BETA
PCR
X-ray
affinity purification
confocal microscopy
transfection

Software Mentioned

Representational Difference Analysis ( RDA )
Adobe Photoshop
Image Pro Express
Blastx

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