DnaK dependence of mutant ethanol oxidoreductases evolved for aerobic function and protective role of the chaperone against protein oxidative damage in Escherichia coli

Proceedings of the National Academy of Sciences of the United States of America
Pedro EchaveE C C Lin

Abstract

The adhE gene of Escherichia coli encodes a multifunctional ethanol oxidoreductase (AdhE) that catalyzes successive reductions of acetyl-CoA to acetaldehyde and then to ethanol reversibly at the expense of NADH. Mutant JE52, serially selected for acquired and improved ability to grow aerobically on ethanol, synthesized an AdhE(A267T/E568K) with two amino acid substitutions that sequentially conferred improved catalytic properties and stability. Here we show that the aerobic growth ability on ethanol depends also on protection of the mutant AdhE against metal-catalyzed oxidation by the chaperone DnaK (a member of the Hsp70 family). No DnaK protection of the enzyme is evident during anaerobic growth on glucose. Synthesis of DnaK also protected E. coli from H2O2 killing under conditions when functional AdhE is not required. Our results therefore suggest that, in addition to the known role of protecting cells against heat stress, DnaK also protects numerous kinds of proteins from oxidative damage.

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Citations

Mar 24, 2006·Archives of Microbiology·C BiancoR Defez
Oct 19, 2011·Applied Microbiology and Biotechnology·Timothy J HanlyMichael A Henson
Mar 10, 2004·Applied and Environmental Microbiology·Eva H HansenLone Gram
May 5, 2005·Applied and Environmental Microbiology·Abram AertsenChris W Michiels
Jun 7, 2005·Journal of Bacteriology·Asa FredrikssonThomas Nyström
Jan 1, 2005·Proceedings of the National Academy of Sciences of the United States of America·Kyu Y RheeCarl F Nathan
Oct 1, 2008·Fungal Genetics and Biology : FG & B·Marta Montero-BarrientosEnrique Monte
Oct 28, 2006·Current Opinion in Microbiology·Asa Fredriksson, Thomas Nyström
Sep 27, 2014·IET Systems Biology·Michael A Henson, Timothy J Hanly
Mar 8, 2005·Research in Microbiology·Jan SermonChris W Michiels
Sep 4, 2004·American Journal of Physiology. Heart and Circulatory Physiology·Jaroslaw KanskiChristian Schöneich
Jun 5, 2003·The Journal of Biological Chemistry·Pedro EchaveJoaquim Ros
Sep 5, 2007·Microbiology·Vineet K SinghNeal R Chamberlain
Dec 1, 2004·EcoSal Plus·R Gary Sawers, David P Clark
Nov 1, 2005·EcoSal Plus·David P Clark, John E Cronan

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