DnaK prevents human insulin amyloid fiber formation on hydrophobic surfaces

Biochemistry
Thomas BalletMarianne Weidenhaupt

Abstract

We have developed a multiwell-based protein aggregation assay to study the kinetics of insulin adsorption and aggregation on hydrophobic surfaces and to investigate the molecular mechanisms involved. Protein-surface interaction progresses in two phases: (1) a lag phase during which proteins adsorb and prefibrillar aggregates form on the material surface and (2) a growth phase during which amyloid fibers form and then are progressively released into solution. We studied the effect of three bacterial chaperones, DnaK, DnaJ, and ClpB, on insulin aggregation kinetics. In the presence of ATP, the simultaneous presence of DnaK, DnaJ, and ClpB allows good protection of insulin against aggregation. In the absence of ATP, DnaK alone is able to prevent insulin aggregation. Furthermore, DnaK binds to insulin adsorbed on hydrophobic surfaces. This process is slowed in the presence of ATP and can be enhanced by the cochaperone DnaJ. The peptide LVEALYL, derived from the insulin B chain, is known to promote fast aggregation in a concentration- and pH-dependent manner in solution. We show that it also shortens the lag phase for insulin aggregation on hydrophobic surfaces. As this peptide is also a known DnaK substrate, our data indicate that ...Continue Reading

References

Jan 1, 1991·Annual Review of Biochemistry·R J Ellis, S M van der Vies
Nov 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·J S McCarty, G C Walker
Nov 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·V SluzkyR Langer
Jan 1, 1990·Methods in Enzymology·C M Stoscheck
Aug 1, 1989·Trends in Biochemical Sciences·R J Ellis, S M Hemmingsen
Jun 20, 1972·Biochemistry·M J Burke, M A Rougvie
Dec 1, 1971·The Biochemical Journal·T L BlundellD Mercola
Nov 3, 1995·The Journal of Biological Chemistry·D Skowyra, S Wickner
Jul 3, 1995·Proceedings of the National Academy of Sciences of the United States of America·K LiberekC Georgopoulos
May 26, 1995·Journal of Molecular Biology·J S McCartyB Bukau
Nov 15, 1996·Journal of Molecular Biology·H TheyssenJ Reinstein
May 1, 1997·Journal of Pharmaceutical Sciences·J BrangeE Rasmussen
Feb 26, 1998·Cell·B Bukau, A L Horwich
Dec 23, 1998·Proceedings of the National Academy of Sciences of the United States of America·W C SuhC A Gross
Nov 26, 1999·Proceedings of the National Academy of Sciences of the United States of America·P GoloubinoffB Bukau
May 10, 2000·The Journal of Biological Chemistry·S DiamantP Goloubinoff
Sep 21, 2000·Current Biology : CB·D A Smith, S E Radford
Feb 28, 2007·Chemical Research in Toxicology·Olga M ViquezWilliam M Valentine
Oct 20, 1992·Biotechnology and Bioengineering·V SluzkyR Langer
Sep 1, 2009·Biochemistry·Tue RasmussenMarco van de Weert
Oct 30, 2009·Proceedings of the National Academy of Sciences of the United States of America·Magdalena I IvanovaDavid Eisenberg
Mar 25, 2010·Pharmaceutical Research·Tue RasmussenWim Jiskoot
Jul 21, 2011·Pharmacognosy Research·Gottumukkala Venkateswara RaoM R Sahoo

❮ Previous
Next ❯

Citations

Jul 7, 2012·Colloids and Surfaces. B, Biointerfaces·Lalit M PandeyMarianne Weidenhaupt
Jul 28, 2015·Langmuir : the ACS Journal of Surfaces and Colloids·Sergio MauriHeike Arnolds
Aug 4, 2015·The Journal of Physical Chemistry. B·Karim ChouchaneMarianne Weidenhaupt
Oct 26, 2018·Biointerphases·Elisa MiglioriniCatherine Picart
Dec 14, 2016·Langmuir : the ACS Journal of Surfaces and Colloids·Thibaut FrachonMarianne Weidenhaupt

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.