DNA/RNA chimera templates improve the emission intensity and target the accessibility of silver nanocluster-based sensors for human microRNA detection

The Analyst
Pratik ShahSeong Wook Yang

Abstract

In recent years microRNAs (miRNAs) have been established as important biomarkers in a variety of diseases including cancer, diabetes, cardiovascular disease, aging, Alzheimer's disease, asthma, autoimmune disease and liver diseases. As a consequence, a variety of monitoring methods for miRNAs have been developed, including a fast and simple method for miRNA detection by exploitation of the unique photoluminescence of DNA-templated silver nanoclusters (DNA/AgNCs). To increase the versatility of the AgNC-based method, we have adopted DNA/RNA chimera templates for AgNC-based probes, allowing response from several human miRNAs which are hardly detectable with DNA-based probes. Here, we demonstrate in detail the power of DNA/RNA chimera/AgNC probes in detecting two human miRNAs, let-7a and miR-200c. The DNA/RNA chimera-based probes are highly efficient to determine the level of miRNAs in several human cell lines.

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Citations

Jul 22, 2016·Molecules and Cells·Seok Keun ChoSeong Wook Yang
Dec 6, 2016·Biomarkers in Medicine·Roberto Fiammengo
Apr 4, 2018·Chemical Society Reviews·Zhaowei ChenXiaogang Qu
Jan 27, 2017·Journal of Movement Disorders·Pratik ShahSeong Wook Yang
Sep 16, 2020·Analytical Methods : Advancing Methods and Applications·Xiangyu FengShuo Wang

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