DNase I-like endonuclease in rat kidney cortex that is activated during ischemia/reperfusion injury.

Journal of the American Society of Nephrology : JASN
Alexei G BasnakianSudhir V Shah

Abstract

Ischemia/reperfusion is known to result in DNA fragmentation and cell death in kidney tubular epithelium, but the endonucleases responsible for this DNA damage have not been identified. DNA substrate gel analysis of extracts from normal rat kidney cortex revealed the presence of a DNase with an apparent molecular mass of 30 to 34 kD. This enzyme is not a dimer of the previously described nuclear 15-kD endonuclease in kidney cells. Partially purified DNase exhibited characteristics similar to those of rat DNase I. The DNase was able to digest circular DNA (endonuclease), required both Ca(2+) and Mg(2+) ions, and was inhibited by Zn(2+) and by aurintricarboxylic acid; it was not inhibited by G-actin. Rat kidneys were subjected to 40 min of ischemia, followed by 0, 1, 4, 16, or 48 h of reperfusion. The activity of the DNase in cytosolic and nuclear extracts, the 200-bp ladder-generating activity, and 3'OH strand breaks in nuclear DNA were simultaneously increased after ischemia, during the first hours of reperfusion. Oxidative DNA damage, measured as 8-hydroxydeoxyguanosine content, did not coincide with endonuclease-generated DNA breaks. Oxidative DNA damage was increased during ischemia and gradually decreased during reperfusion...Continue Reading

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Citations

Jul 23, 2003·Journal of the American Society of Nephrology : JASN·Joseph V Bonventre, Joel M Weinberg
Feb 5, 2003·Experimental Cell Research·Sergey Ya ProskuryakovVladimir L Gabai
Feb 8, 2003·Antioxidants & Redox Signaling·Alexei G BasnakianSudhir V Shah
Jun 5, 2021·Journal of Cellular and Molecular Medicine·Alexei G Basnakian, Christopher L Moore
Jun 20, 2003·Current Opinion in Nephrology and Hypertension·George JaradJeffrey R Schelling
Dec 18, 2003·American Journal of Physiology. Renal Physiology·Qingqing WeiZheng Dong
Apr 29, 2004·American Journal of Physiology. Renal Physiology·Qingqing WeiZheng Dong
Jul 29, 2004·American Journal of Physiology. Renal Physiology·Shenyang LiDidier Portilla
Jul 16, 2004·Kidney International·Gur P KaushalSudhir V Shah
Jan 14, 2005·Journal of the American Society of Nephrology : JASN·Alexei G BasnakianSudhir V Shah
Mar 10, 2005·American Journal of Physiology. Renal Physiology·Craig BrooksZheng Dong
Aug 18, 2005·American Journal of Physiology. Renal Physiology·Qingqing WeiZheng Dong
Oct 19, 2006·Experimental Cell Research·Alexei G BasnakianSudhir V Shah
Aug 7, 2007·Journal of the American Society of Nephrology : JASN·Xiaoyan YinAlexei G Basnakian
Oct 4, 2007·Biochimica Et Biophysica Acta·Yoshihiko KominatoToshihiro Yasuda
Dec 22, 2007·Nephron. Experimental Nephrology·Jianzhong WangQuan Hong
Sep 24, 2009·Radiation Research·Eugene O ApostolovAlexei G Basnakian

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