PMID: 835Nov 1, 1975

DNases and RNases of Misgurnus fossilis ovocytes

Ukrainskiĭ biokhimicheskiĭ zhurnal
S I Kusen', P S Oleshko

Abstract

The pH optima were determined for DNases and RNases of the loach eggs. For DNases they are 5.6 and 7.6 and for RNases - 5.2 and 7.2. It is established that Ca++ activates, and Fe++ has not effect on the activity of acid and alkaline DNases, while Mg++, Mn++ and especially Co++, Zn++, Cd++, Cu++ have an inhibitory effect on them. The activities of RNases is stimulated by Ca++ and Fe++, and inhibited by Zn++, Co++, Cd++ and Cu++. Iones Mg++ and Mn++ do not affect these activities. Localization of the above mentioned enzymes was studied by means of differential centrifugation of egg homogenates. Acid DNase is concentrated only in postmicrosomal supernatant liquid, its activity being inhibited in the presence of the nucleomitochondrial and microsomal fractions. Acid RNase is also localized predominantly in postmicrosomal supernatant fraction. Alkaline DNase is found to a great extent in nucleomitochondrial fraction, and alkaline RNase - in postmicrosomal one.

Related Concepts

Calcium
Cations, Divalent
Alkaline DNase
Enzyme Activation
Hydrogen-Ion Concentration
Oocytes
Ovum
Alkaline Ribonuclease

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