DNAzyme-Powered Three-Dimensional DNA Walker Nanoprobe for Detection Amyloid β-Peptide Oligomer in Living Cells and in Vivo

Analytical Chemistry
Yiming YinFenglei Gao

Abstract

Amyloid β-peptide oligomer (AβO) is widely acknowledged as the promising biomarker for the diagnosis of Alzheimer's disease (AD). In this work, we designed a three-dimensional (3D) DNA walker nanoprobe for AβO detection and real-time imaging in living cells and in vivo. The presence of AβO triggered the DNAzyme walking strand to cleave the fluorophore (TAMRA)-labeled substrate strand modified on the gold nanoparticle (AuNP) surface and release TAMRA-labeled DNA fragment, resulting in the recovery of fluorescent signal. The entire process was autonomous and continuous, without external fuel strands or protease, and finally produced plenty of TAMRA fluorescence, achieving signal amplification effect. The nanoprobe enabled the quantitative detection of AβO in vitro, and the limit of detection was 22.3 pM. Given the good biocompatibility of 3D DNA walker nanoprobe, we extended this enzyme-free signal amplification method to real-time imaging of AβO. Under the microscope, nanoprobe accurately located and visualized the distribution of AβO in living cells. Moreover, in vivo imaging results showed that our nanoprobe could be used to effectively distinguish the AD mice from the wild-type mice. This nanoprobe with the advantages of grea...Continue Reading

References

Sep 16, 2004·Journal of the American Chemical Society·Ye Tian, Chengde Mao
Jul 26, 2008·Nature Nanotechnology·Jonathan Bath, Andrew J Turberfield
Aug 20, 2009·Chemical Society Reviews·Arvi Rauk
Sep 16, 2009·Accounts of Chemical Research·Xiaohong Fang, Weihong Tan
Oct 15, 2009·Journal of the American Chemical Society·Bin-Cheng YinCong-Cong Xie
Feb 16, 2010·Archives of Biochemistry and Biophysics·Paulius CizasVilmante Borutaite
May 8, 2010·The Analyst·Ryan PicouS Douglass Gilman
Feb 22, 2011·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Ryan A PicouS Douglass Gilman
Feb 3, 2012·Angewandte Chemie·Mingxu YouWeihong Tan
Jun 16, 2012·Analytical Chemistry·Kaori TsukakoshiKazunori Ikebukuro
Jul 17, 2012·Chemical Reviews·Kasper P Kepp
Oct 2, 2012·Analytical Biochemistry·Kim A BrugginkMarcel M Verbeek
Dec 1, 2012·Nano Letters·Xiaoqing LiuItamar Willner
Jan 12, 2013·Alzheimer's & Dementia : the Journal of the Alzheimer's Association·Martin PrinceCleusa P Ferri
Jan 15, 2013·Journal of Alzheimer's Disease : JAD·Lei Wang-DietrichDieter Willbold
Jul 4, 2013·Nature Communications·Minghui LiuHao Yan
Aug 6, 2013·Accounts of Chemical Research·Changqin DingYang Tian
Oct 26, 2013·Journal of Alzheimer's Disease : JAD·Takehiro KikoTeruo Miyazawa
Dec 25, 2013·Genes & Nutrition·Johnathan R Nuttall, Patricia I Oteiza
Jan 13, 2015·Advances in Medical Sciences·Piotr LewczukJohannes Kornhuber
Jul 8, 2015·Journal of the American Chemical Society·Tae-Gon ChaJong Hyun Choi
Mar 8, 2016·ACS Applied Materials & Interfaces·Yu YangLidong Li
May 24, 2016·EBioMedicine·Urmi SenguptaRakez Kayed
Jul 19, 2017·ACS Nano·Cheulhee JungAndrew D Ellington
Sep 16, 2017·Science·Anupama J ThubagereLulu Qian
Feb 20, 2018·ACS Applied Materials & Interfaces·Caifeng DingXiliang Luo

❮ Previous
Next ❯

Citations

Sep 22, 2020·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Ling HuXiao-Bing Zhang
Jan 20, 2021·Wiley Interdisciplinary Reviews. Nanomedicine and Nanobiotechnology·Lingxiao ZhangDaishun Ling
Mar 26, 2021·Analytical Chemistry·Mingyuan DuZhike He
Apr 27, 2021·Advanced Science·Liangcan HeXiaoyuan Chen
Jun 18, 2021·Advanced Drug Delivery Reviews·Xi HuDaishun Ling
Jun 20, 2021·Advanced Drug Delivery Reviews·Lingxiao ZhangFangyuan Li
Jul 20, 2021·ACS Biomaterials Science & Engineering·Fantian ZengJian Yang
Aug 28, 2021·Journal of Biomedical Nanotechnology·Xiao-Lin WangHan-Jun Li
Nov 24, 2021·Analytical Methods : Advancing Methods and Applications·Chaohui ChenLong Chen

❮ Previous
Next ❯

Related Concepts

Related Feeds

Alzheimer's Disease: Neuroimaging

Neuroimaging can help identify pathological hallmarks of Alzheimer's disease (AD). Here is the latest research on neuroimaging modalities, including magnetic resonance imaging and positron emission tomography, in AD.

Alzheimer's Disease: Amyloid Beta

Alzheimer's disease is a neurodegenerative disease associated with the accumulation of amyloid plaques in the brain; these plaques are comprised of amyloid beta deposits. Here is the latest research in this field.